Insights into Wound Closure on Two Biopolyesters-Polylactide and Polyhydroxyoctanoate

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2020 Jun 25

PMID 32575761

Citations 7

Authors

Tomasz Witko

Daria Solarz

Karolina Feliksiak

Katarzyna Harazna

Zenon Rajfur

Maciej Guzik

Affiliations

Soon will be listed here.

Abstract

Two bio-based polymers have been compared in this study, namely: polylactide (PLA) and polyhydroxyoctanoate (PHO). Due to their properties such as biocompatibility, and biointegrity they are considered to be valuable materials for medical purposes, i.e., creating scaffolds or wound dressings. Presented biopolymers were investigated for their impact on cellular migration strategies of mouse embryonic fibroblasts (MEF) 3T3 cell line. Advanced microscopic techniques, including confocal microscopy and immunofluorescent protocols, enabled the thorough analysis of the cell shape and migration. Application of wound healing assay combined with dedicated software allowed us to perform quantitative analysis of wound closure dynamics. The outcome of the experiments demonstrated that the wound closure dynamics for PLA differs from PHO. Single fibroblasts grown on PLA moved 1.5-fold faster, than those migrating on the PHO surface. However, when a layer of cells was considered, the wound closure was by 4.1 h faster for PHO material. The accomplished work confirms the potential of PLA and PHO as excellent candidates for medical applications, due to their properties that propagate cell migration, vitality, and proliferation-essential cell processes in the healing of damaged tissues.

Citing Articles

Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/P(3HO) scaffolds for tissue engineering applications.

Solarz D, Witko T, Karcz R, Malagurski I, Ponjavic M, Levic S RSC Adv. 2023; 13(34):24112-24128.

PMID: 37577093 PMC: 10415749. DOI: 10.1039/d3ra03021k.

In Vitro and In Vivo Characterization Methods for Evaluation of Modern Wound Dressings.

Ahmad N Pharmaceutics. 2023; 15(1).

PMID: 36678671 PMC: 9864730. DOI: 10.3390/pharmaceutics15010042.

The Influence of Novel, Biocompatible, and Bioresorbable Poly(3-hydroxyoctanoate) Dressings on Wound Healing in Mice.

Seta M, Harazna K, Kasarello K, Solarz-Keller D, Cudnoch-Jedrzejewska A, Witko T Int J Mol Sci. 2022; 23(24).

PMID: 36555799 PMC: 9785414. DOI: 10.3390/ijms232416159.

Polyhydroxyalkanoates, bacterially synthesized polymers, as a source of chemical compounds for the synthesis of advanced materials and bioactive molecules.

Guzik M Appl Microbiol Biotechnol. 2021; 105(20):7555-7566.

PMID: 34536102 PMC: 8502142. DOI: 10.1007/s00253-021-11589-0.

Vimentin Cytoskeleton Architecture Analysis on Polylactide and Polyhydroxyoctanoate Substrates for Cell Culturing.

Feliksiak K, Solarz D, Guzik M, Zima A, Rajfur Z, Witko T Int J Mol Sci. 2021; 22(13).

PMID: 34201927 PMC: 8268722. DOI: 10.3390/ijms22136821.

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