Circulating MiRNAs in Small Extracellular Vesicles Secreted by a Human Melanoma Xenograft in Mouse Brains

Overview

Journal Cancers (Basel)

Publisher MDPI

Specialty Oncology

Date 2020 Jun 25

PMID 32575666

Citations 8

Authors

Loredana Guglielmi

Marta Nardella

Carla Musa

Ingrid Cifola

Manuela Porru

Beatrice Cardinali

Ilaria Iannetti

Chiara Di Pietro

Giulia Bolasco

Valentina Palmieri

Laura Vilardo

Nicolo Panini

Fabrizio Bonaventura

Massimiliano Papi

Ferdinando Scavizzi

Marcello Raspa

Carlo Leonetti

Germana Falcone

Armando Felsani

Igea DAgnano

Affiliations

Soon will be listed here.

Abstract

The identification of liquid biomarkers remains a major challenge to improve the diagnosis of melanoma patients with brain metastases. Circulating miRNAs packaged into tumor-secreted small extracellular vesicles (sEVs) contribute to tumor progression. To investigate the release of tumor-secreted miRNAs by brain metastasis, we developed a xenograft model where human metastatic melanoma cells were injected intracranially in nude mice. The comprehensive profiles of both free miRNAs and those packaged in sEVs secreted by the melanoma cells in the plasma demonstrated that most (80%) of the sEV-associated miRNAs were also present in serum EVs from a cohort of metastatic melanomas, included in a publicly available dataset. Remarkably, among them, we found three miRNAs (miR-224-5p, miR-130a-3p and miR-21-5p) in sEVs showing a trend of upregulation during melanoma progression. Our model is proven to be valuable for identifying miRNAs in EVs that are unequivocally secreted by melanoma cells in the brain and could be associated to disease progression.

Citing Articles

Plant-derived extracellular nanovesicles: a promising biomedical approach for effective targeting of triple negative breast cancer cells.

Cui L, Perini G, Augello A, Palmieri V, De Spirito M, Papi M Front Bioeng Biotechnol. 2024; 12:1390708.

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Modulating Effects of Cancer-Derived Exosomal miRNAs and Exosomal Processing by Natural Products.

Chuang Y, Tang J, Shiau J, Yen C, Chang F, Yang K Cancers (Basel). 2023; 15(1).

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miRNome and Proteome Profiling of Small Extracellular Vesicles Secreted by Human Glioblastoma Cell Lines and Primary Cancer Stem Cells.

Cifola I, Fratini F, Cardinali B, Palmieri V, Gatti G, Selmi T Biomedicines. 2022; 10(8).

PMID: 36009432 PMC: 9405730. DOI: 10.3390/biomedicines10081886.

Liquid biopsies to occult brain metastasis.

Rehman A, Khan P, Maurya S, Siddiqui J, Santamaria-Barria J, Batra S Mol Cancer. 2022; 21(1):113.

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Opportunities Offered by Graphene Nanoparticles for MicroRNAs Delivery for Amyotrophic Lateral Sclerosis Treatment.

Niccolini B, Palmieri V, De Spirito M, Papi M Materials (Basel). 2022; 15(1).

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