Expression Patterns of Innate Immunity-related Genes in Response to Polyinosinic:polycytidylic Acid (poly[I:C]) Stimulation in DF-1 Chicken Fibroblast Cells

Overview

Journal J Anim Sci Technol

Date 2020 Jun 23

PMID 32568266

Citations 6

Authors

Hyun-Jun Jang

Ki-Duk Song

Affiliations

Soon will be listed here.

Abstract

Polyinosinic:polycytidylic acid (poly[I:C]) can stimulate Toll-like receptor 3 (TLR3) signaling pathways. In this study, DF-1 cells were treated with poly(I:C) at various concentrations and time points to examine the comparative expression patterns of innate immune response genes. The viability of DF-1 cells decreased from 77.41% to 38.68% when cells were treated different dose of poly(I:C) from 0.1 µg/mL to 100 µg/mL for 24 h respectively. The expressions of , and were increased in dose- and time-dependent manners by poly(I:C) treatment. On the contrary, the expression patterns of (), , (), (), and were varied; and were increasingly expressed whereas the expressions of and were decreased in a dose-dependent manner after they were early induced. In time-dependent analysis, expression was significantly upregulated from 3 h to 24 h, whereas and expressions settled down from 6 h to 24 h after poly(I:C) treatment although they were induced at early time from 1 h to 3 h. Poly(I:C) treatment rapidly increased the expression of from 3 h to 6 h with a plateau at 6 h and then the expression of was dramatically decreased until 24 h after poly(I:C) treatment although the expression level was still higher than the non-treated control. These results may provide the basis for understanding host response to viral infection and its mimicry system in chickens.

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