Infectious Risk of the Hospital Environment in the Center of Morocco: A Case of Care Unit Surfaces

Overview

Journal Scientifica (Cairo)

Publisher Wiley

Specialty Biology

Date 2020 Jun 23

PMID 32566360

Citations 2

Authors

Samira Jaouhar

Abdelhakim El Ouali Lalami

Khadija Ouarrak

Jawad Bouzid

Mohammed Maoulouaa

Khadija Bekhti

Affiliations

Soon will be listed here.

Abstract

Background: Equipment and hospital surfaces constitute a microbial reservoir that can contaminate hospital users and thus create an infectious risk. The aim of this work, which was carried out for the first time at a hospital in Meknes (regional hospital in the center of Morocco), is to evaluate the microbiological quality of surfaces and equipment in three potential risk areas (burn unit, operating room, and sterilization service).

Methods: This study was carried out over a period of 4 months (February-May 2017). A total of 60 samples were taken by swabbing according to the standard (ISO/DIS 14698-1 (2004)) in an environment of dry area and equipment after biocleaning. Isolation and identification were performed according to conventional bacteriological methods and by microscopic observation for fungi.

Results: The study showed that 40% of surface samples were contaminated after biocleaning. The burn unit recorded a percentage of 70% contamination ( value <0.001), 13% for the sterilization service, and 7% for the operating room. 89% of the isolates were identified as Gram-positive bacteria against 11% for fungi ( value <0.001). Bacterial identification showed coagulase-negative staphylococci (32%), spp. (16%), (8%), and oxidase-negative Gram-positive bacillus (40%) while fungal identification showed ( = 2) and ( = 1).

Conclusion: To control the infectious risk related to equipment and hospital surfaces, it would be necessary to evaluate the disinfection protocol applied in these units.

Citing Articles

Microbiological Monitoring of the Environment Using the "Association Rules" Approach and Disinfection Procedure Evaluation in a Hospital Center in Morocco.

Flouchi R, Elmniai A, El Far M, Touzani I, El Hachlafi N, Fikri-Benbrahim K J Environ Public Health. 2021; 2021:7682042.

PMID: 34285698 PMC: 8275422. DOI: 10.1155/2021/7682042.

Evaluation of Antibacterial Activity of Three Quaternary Ammonium Disinfectants on Different Germs Isolated from the Hospital Environment.

Ramzi A, Oumokhtar B, Ez Zoubi Y, Filali Mouatassem T, Benboubker M, El Ouali Lalami A Biomed Res Int. 2020; 2020:6509740.

PMID: 33381566 PMC: 7749782. DOI: 10.1155/2020/6509740.

References

Vandini A, Temmerman R, Frabetti A, Caselli E, Antonioli P, Balboni P . Hard surface biocontrol in hospitals using microbial-based cleaning products. PLoS One. 2014; 9(9):e108598. PMC: 4178175. DOI: 10.1371/journal.pone.0108598. View

Carling P, Briggs J, Hylander D, Perkins J . An evaluation of patient area cleaning in 3 hospitals using a novel targeting methodology. Am J Infect Control. 2006; 34(8):513-9. DOI: 10.1016/j.ajic.2005.09.001. View

Araiza J, Canseco P, Bonifaz A . Otomycosis: clinical and mycological study of 97 cases. Rev Laryngol Otol Rhinol (Bord). 2007; 127(4):251-4. View

Manian F, Griesenauer S, Senkel D, Setzer J, Doll S, Perry A . Isolation of Acinetobacter baumannii complex and methicillin-resistant Staphylococcus aureus from hospital rooms following terminal cleaning and disinfection: can we do better?. Infect Control Hosp Epidemiol. 2011; 32(7):667-72. DOI: 10.1086/660357. View

Hirai Y . Survival of bacteria under dry conditions; from a viewpoint of nosocomial infection. J Hosp Infect. 1991; 19(3):191-200. DOI: 10.1016/0195-6701(91)90223-u. View

Donskey C . Does improving surface cleaning and disinfection reduce health care-associated infections?. Am J Infect Control. 2013; 41(5 Suppl):S12-9. DOI: 10.1016/j.ajic.2012.12.010. View

Person A, Chudgar S, Norton B, Tong B, Stout J . Aspergillus niger: an unusual cause of invasive pulmonary aspergillosis. J Med Microbiol. 2010; 59(Pt 7):834-838. PMC: 3052473. DOI: 10.1099/jmm.0.018309-0. View

Bouguenoun W, Bakour S, Bentorki A, Al Bayssari C, Merad T, Rolain J . Molecular epidemiology of environmental and clinical carbapenemase-producing Gram-negative bacilli from hospitals in Guelma, Algeria: Multiple genetic lineages and first report of OXA-48 in Enterobacter cloacae. J Glob Antimicrob Resist. 2016; 7:135-140. DOI: 10.1016/j.jgar.2016.08.011. View

Kramer A, Schwebke I, Kampf G . How long do nosocomial pathogens persist on inanimate surfaces? A systematic review. BMC Infect Dis. 2006; 6:130. PMC: 1564025. DOI: 10.1186/1471-2334-6-130. View

10.

Loudon K, Coke A, Burnie J, Shaw A, Oppenheim B, Morris C . Kitchens as a source of Aspergillus niger infection. J Hosp Infect. 1996; 32(3):191-8. DOI: 10.1016/s0195-6701(96)90145-0. View

11.

Alberti C, Bouakline A, Ribaud P, Lacroix C, Rousselot P, Leblanc T . Relationship between environmental fungal contamination and the incidence of invasive aspergillosis in haematology patients. J Hosp Infect. 2001; 48(3):198-206. DOI: 10.1053/jhin.2001.0998. View

12.

Chu V, Woods C, Miro J, Hoen B, Cabell C, Pappas P . Emergence of coagulase-negative staphylococci as a cause of native valve endocarditis. Clin Infect Dis. 2008; 46(2):232-42. DOI: 10.1086/524666. View

13.

Boyce J . Environmental contamination makes an important contribution to hospital infection. J Hosp Infect. 2007; 65 Suppl 2:50-4. DOI: 10.1016/S0195-6701(07)60015-2. View

14.

Al Kadi A, Salati S . Hand Hygiene Practices among Medical Students. Interdiscip Perspect Infect Dis. 2012; 2012:679129. PMC: 3457633. DOI: 10.1155/2012/679129. View

15.

Morello-Frosch R, Brody J, Brown P, Altman R, Rudel R, Perez C . Toxic ignorance and right-to-know in biomonitoring results communication: a survey of scientists and study participants. Environ Health. 2009; 8:6. PMC: 2654440. DOI: 10.1186/1476-069X-8-6. View

16.

Sergent A, Slekovec C, Pauchot J, Jeunet L, Bertrand X, Hocquet D . Bacterial contamination of the hospital environment during wound dressing change. Orthop Traumatol Surg Res. 2012; 98(4):441-5. DOI: 10.1016/j.otsr.2012.02.005. View

17.

Amazian K, Rossello J, Castella A, Sekkat S, Terzaki S, Dhidah L . [Prevalence of nosocomial infections in 27 hospitals in the Mediterranean region]. East Mediterr Health J. 2011; 16(10):1070-8. View

18.

El Rhazi K, Elfakir S, Berraho M, Tachfouti N, Serhier Z, Kanjaa C . [Prevalence and risk factors for nosocomial infections in Hassan II University Hospital, Fes, Morocco]. East Mediterr Health J. 2007; 13(1):56-63. View

19.

Jroundi I, Khoudri I, Azzouzi A, Zeggwagh A, Benbrahim N, Hassouni F . Prevalence of hospital-acquired infection in a Moroccan university hospital. Am J Infect Control. 2007; 35(6):412-6. DOI: 10.1016/j.ajic.2006.06.010. View

20.

Munoz-Price L, Birnbach D, Lubarsky D, Arheart K, Fajardo-Aquino Y, Rosalsky M . Decreasing operating room environmental pathogen contamination through improved cleaning practice. Infect Control Hosp Epidemiol. 2012; 33(9):897-904. DOI: 10.1086/667381. View