Ginsenoside Rg1 Improves Differentiation by Inhibiting Senescence of Human Bone Marrow Mesenchymal Stem Cell Via GSK-3 and -Catenin

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2020 Jun 23

PMID 32565825

Citations 11

Authors

Ziling Wang

Rong Jiang

Lu Wang

Xiongbin Chen

Yue Xiang

Linbo Chen

Minghe Xiao

Li Ling

Yaping Wang

Affiliations

Soon will be listed here.

Abstract

Objectives: To demonstrate the effect of Ginsenoside Rg1 on the differentiation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Subsequently, a rational mechanism for the detection of Rg1 which affects mesenchymal stem cell differentiation was explored.

Methods: Flow cytometry is used for cell identification. The differentiation ability of hBM-MSCs was studied by differentiation culture. SA--gal staining is used to detect cell senescence levels. Western blot and immunofluorescence were used to determine protein expression levels. RT-qPCR is used to detect mRNA expression levels.

Results: Rg1 regulates the differentiation of hBM-MSCs. Differentiation culture analysis showed that Rg1 promoted cells to osteogenesis and chondrogenesis. Western blot results showed that Rg1 regulated the overactivation of the -catenin signaling pathway and significantly adjusted the phosphorylation of GSK-3. GSK-3 inhibitor (Licl) significantly increased Rg1-induced phosphorylation of GSK-3, which in turn reduced Rg1-induced differentiation of hBM-MSCs.

Conclusion: Ginsenoside Rg1 can reduce the excessive activation of the Wnt pathway in senescent cells by inhibiting the phosphorylation of GSK-3 and regulate the mesenchymal stem cell differentiation ability.

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