Enhanced Sensitivity of VEGF Detection Using Catalase-mediated Chemiluminescence Immunoassay Based on CdTe QD/HO System

Overview

Journal J Nanobiotechnology

Publisher Biomed Central

Specialty Biotechnology

Date 2020 Jun 20

PMID 32552818

Citations 4

Authors

Fahimeh Ghavamipour

Hossein Rahmani

Maryam Shanehsaz

Khosro Khajeh

Manouchehr Mirshahi

Reza H Sajedi

Affiliations

Soon will be listed here.

Abstract

Background: Since vascular endothelial growth factor (VEGF) is a significant regulator of cancer angiogenesis, it is essential to develop a technology for its sensitive detection. Herein, we sensitized a chemiluminescence (CL) immunoassay through the combination of HO-sensitive TGA-CdTe quantum dot (QD) as signal transduction, dextran as a cross-linker to prepare enzyme-labeled antigen and the ultrahigh bioactivity of catalase (CAT) as reporter enzyme.

Results: Under the optimized experimental conditions, the chemiluminescence enzyme-linked immunosorbent assay (CL-ELISA) method can detect VEGF in the excellent linear range of 2-35,000 pg mL, with a detection limit (S/N = 3) of 0.5 pg mL which was approximately ten times lower than the commercial colorimetric immunoassay. This proposed method has been successfully applied to the clinical determination of VEGF in the human serum samples, and the results illustrated an excellent correlation with the conventional ELISA method (R = 0.997). The suitable recovery rate of the method in the serum ranged from 97 to 107%, with a relative standard deviation of 1.2% to 13.4%.

Conclusions: The novel immunoassay proposes a highly sensitive, specific, and stable method for very low levels detection of VEGF that can be used in the primary diagnosis of tumors. With the well-designed sensing platform, this approach has a broad potential to be applied for quantitative analysis of numerous disease-related protein biomarkers for which antibodies are available.

Citing Articles

A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF) based on anti-VEGF-iron oxide nanoparticle conjugation.

Ceylan H, Kirbay F, Yazgan I, Elibol M Mikrochim Acta. 2024; 191(3):133.

PMID: 38353782 PMC: 10867064. DOI: 10.1007/s00604-024-06228-0.

Emerging Biosensing Methods to Monitor Lung Cancer Biomarkers in Biological Samples: A Comprehensive Review.

Chinnappan R, Mir T, Alsalameh S, Makhzoum T, Alzhrani A, Alnajjar K Cancers (Basel). 2023; 15(13).

PMID: 37444523 PMC: 10341261. DOI: 10.3390/cancers15133414.

A systematic review and meta-analysis of preanalytical factors and methodological differences influencing the measurement of circulating vascular endothelial growth factor.

Sjobom U, Nilsson A, Gyllensten H, Hellstrom A, Lofqvist C PLoS One. 2022; 17(7):e0270232.

PMID: 35793362 PMC: 9258884. DOI: 10.1371/journal.pone.0270232.

Aptamer-modified DNA tetrahedra-gated metal-organic framework nanoparticle carriers for enhanced chemotherapy or photodynamic therapy.

Zhang P, Fischer A, Ouyang Y, Wang J, Sohn Y, Nechushtai R Chem Sci. 2021; 12(43):14473-14483.

PMID: 34880998 PMC: 8580039. DOI: 10.1039/d1sc04229g.

References

Giljohann D, Mirkin C . Drivers of biodiagnostic development. Nature. 2009; 462(7272):461-4. PMC: 3936986. DOI: 10.1038/nature08605. View

Salven P, Perhoniemi V, Tykka H, Maenpaa H, Joensuu H . Serum VEGF levels in women with a benign breast tumor or breast cancer. Breast Cancer Res Treat. 1999; 53(2):161-6. DOI: 10.1023/a:1006178517505. View

Gao Z, Xu M, Hou L, Chen G, Tang D . Magnetic bead-based reverse colorimetric immunoassay strategy for sensing biomolecules. Anal Chem. 2013; 85(14):6945-52. DOI: 10.1021/ac401433p. View

Wulfkuhle J, Liotta L, Petricoin E . Proteomic applications for the early detection of cancer. Nat Rev Cancer. 2003; 3(4):267-75. DOI: 10.1038/nrc1043. View

de la Rica R, Stevens M . Plasmonic ELISA for the ultrasensitive detection of disease biomarkers with the naked eye. Nat Nanotechnol. 2012; 7(12):821-4. DOI: 10.1038/nnano.2012.186. View

Shan S, He Z, Mao S, Jie M, Yi L, Lin J . Quantitative determination of VEGF165 in cell culture medium by aptamer sandwich based chemiluminescence assay. Talanta. 2017; 171:197-203. DOI: 10.1016/j.talanta.2017.04.057. View

Nakahara H, Song J, Sugimoto M, Hagihara K, Kishimoto T, Yoshizaki K . Anti-interleukin-6 receptor antibody therapy reduces vascular endothelial growth factor production in rheumatoid arthritis. Arthritis Rheum. 2003; 48(6):1521-9. DOI: 10.1002/art.11143. View

Cho H, Yeh E, Sinha R, Laurence T, Bearinger J, Lee L . Single-step nanoplasmonic VEGF165 aptasensor for early cancer diagnosis. ACS Nano. 2012; 6(9):7607-14. PMC: 3458122. DOI: 10.1021/nn203833d. View

Medintz I, Stewart M, Trammell S, Susumu K, Delehanty J, Mei B . Quantum-dot/dopamine bioconjugates function as redox coupled assemblies for in vitro and intracellular pH sensing. Nat Mater. 2010; 9(8):676-84. DOI: 10.1038/nmat2811. View

10.

Loureiro R, DAmore P . Transcriptional regulation of vascular endothelial growth factor in cancer. Cytokine Growth Factor Rev. 2005; 16(1):77-89. DOI: 10.1016/j.cytogfr.2005.01.005. View

11.

Abdelhamid H, Wu H . Probing the interactions of chitosan capped CdS quantum dots with pathogenic bacteria and their biosensing application. J Mater Chem B. 2020; 1(44):6094-6106. DOI: 10.1039/c3tb21020k. View

12.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

13.

Ghavamipour F, Shahangian S, Sajedi R, Arab S, Mansouri K, Aghamaali M . Development of a highly-potent anti-angiogenic VEGF8-109 heterodimer by directed blocking of its VEGFR-2 binding site. FEBS J. 2014; 281(19):4479-94. DOI: 10.1111/febs.12956. View

14.

Kulasingam V, Diamandis E . Strategies for discovering novel cancer biomarkers through utilization of emerging technologies. Nat Clin Pract Oncol. 2008; 5(10):588-99. DOI: 10.1038/ncponc1187. View

15.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

16.

Hsu C, Lien C, Wang C, Harroun S, Huang C, Chang H . Immobilization of aptamer-modified gold nanoparticles on BiOCl nanosheets: Tunable peroxidase-like activity by protein recognition. Biosens Bioelectron. 2015; 75:181-7. DOI: 10.1016/j.bios.2015.08.049. View

17.

Xu W, Xiong Y, Lai W, Xu Y, Li C, Xie M . A homogeneous immunosensor for AFB1 detection based on FRET between different-sized quantum dots. Biosens Bioelectron. 2014; 56:144-50. DOI: 10.1016/j.bios.2014.01.007. View

18.

Ugurel S, Rappl G, Tilgen W, Reinhold U . Increased serum concentration of angiogenic factors in malignant melanoma patients correlates with tumor progression and survival. J Clin Oncol. 2001; 19(2):577-83. DOI: 10.1200/JCO.2001.19.2.577. View

19.

Lin C, Wei K, Liao S, Huang C, Sun C, Wu P . A reusable magnetic graphene oxide-modified biosensor for vascular endothelial growth factor detection in cancer diagnosis. Biosens Bioelectron. 2014; 67:431-7. DOI: 10.1016/j.bios.2014.08.080. View

20.

Detmar M . Evidence for vascular endothelial growth factor (VEGF) as a modifier gene in psoriasis. J Invest Dermatol. 2004; 122(1):xiv-xv. DOI: 10.1046/j.0022-202X.2003.22140.x. View