Laboratory Management for SARS-CoV-2 Detection: a User-friendly Combination of the Heat Treatment Approach and Rt-Real-time PCR Testing

Overview

Journal Emerg Microbes Infect

Specialty Infectious Diseases

Date 2020 Jun 20

PMID 32552549

Citations 25

Authors

Fabiola Mancini

Fabrizio Barbanti

Maria Scaturro

Giulia Errico

Angelo Iacobino

Antonino Bella

Flavia Riccardo

Giulia Marsili

Paola Stefanelli

Patrizio Pezzotti

Giovanni Rezza

Alessandra Ciervo

Affiliations

Soon will be listed here.

Abstract

The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.

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