Intestinal Cestodes of Chicken Are Effectively Killed by Quinoline-rich Extract of

Background And Aim: is used for the treatment of intestinal helminth infections in Mizo traditional medicine. In spite of a variety of drugs developed for helminthiases, an entirely safe and absolutely effective drug is still lacking, so much so that infections remain a major problem in human and animal welfare. In this study, we attempted to substantiate as an anticestodal agent.

Materials And Methods: The aqueous extract of the aerial parts of was prepared and from it a bioactive fraction was obtained using column chromatography. Chemical analyses were done using thin-layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS). Helminth survival test was performed on an intestinal cestode, . Structural effects on the cestode were examined under scanning electron microscopy.

Results: From the bioactive fraction of extract, TLC indicated the presence of an aromatic quinone, which was identified using GC-MS as a quinoline derivative (2,2,4-trimethyl-1,2-dihydroquinoline having a retention time of 24.97 min and chemical formula of CHN). The quinoline-rich fraction showed concentration-dependent activity against as that of albendazole. Scanning electron microscopy of the treated cestode revealed classic anthelmintic effects such as tegumental shrinkage and damage of surface organs. The scolex was shrunk, suckers were degenerated with disintegrated spines, and rostellum was completely collapsed. There were severe damages on the tegument and formation of pit-like scars on the proglottids.

Conclusion: The efficacy of extract and structural damages it caused on the cestode indicates that it is a potential source of anthelmintic agent and that 2,2,4-trimethyl-1,2-dihydroquinoline contributes to its antiparasitic activity.