Pre-Growth Culture Conditions Affect Type 1 Fimbriae-Dependent Adhesion of

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2020 Jun 18

PMID 32545652

Citations 3

Authors

Beata Klasa

Anna Ewa Kedzierska

Krzysztof Grzymajlo

Affiliations

Soon will be listed here.

Abstract

Among various fimbrial structures used by to colonize host tissues, type 1 fimbriae (T1F) are among the most extensively studied. Although some experiments have shown the importance of T1F in the initial stages of infection, their exact role in the infection process is not fully known. We suggested that different outcomes of T1F investigations were due to the use of different pre-infection growth conditions for the induction of the T1F. We utilized qPCR, flow cytometry, and a wide range of adhesion assays to investigate Choleraesuis and Typhimurium adhesion in the context of T1F expression. We demonstrated that T1F expression was highly dependent on the pre-infection growth conditions. These growth conditions yielded T1F+ and T1F- populations of and, therefore, could be a factor influencing host cell interactions. We supported this conclusion by showing that increased levels of T1F expression directly correlated with higher levels of adherence to the intestinal epithelial IPEC-J2 cell line.

Citing Articles

The Game for Three: -Host-Microbiota Interaction Models.

Grzymajlo K Front Microbiol. 2022; 13:854112.

PMID: 35516427 PMC: 9062650. DOI: 10.3389/fmicb.2022.854112.

Identifying Bacterial Lineages in Salmonella by Flow Cytometry.

Fernandez-Fernandez R, Olivenza D, Sanchez-Romero M EcoSal Plus. 2022; 10(1):eESP00182021.

PMID: 35148202 PMC: 10729938. DOI: 10.1128/ecosalplus.ESP-0018-2021.

Identification of Natural Mutations Responsible for Altered Infection Phenotypes of Salmonella enterica Clinical Isolates by Using Cell Line Infection Screens.

Kolenda R, Burdukiewicz M, Wimonc M, Aleksandrowicz A, Ali A, Szabo I Appl Environ Microbiol. 2020; 87(2).

PMID: 33127819 PMC: 7783345. DOI: 10.1128/AEM.02177-20.

References

Grzymajlo K, Ugorski M, Kolenda R, Kedzierska A, Kuzminska-Bajor M, Wieliczko A . FimH adhesin from host unrestricted Salmonella Enteritidis binds to different glycoprotein ligands expressed by enterocytes from sheep, pig and cattle than FimH adhesins from host restricted Salmonella Abortus-ovis, Salmonella Choleraesuis and.... Vet Microbiol. 2013; 166(3-4):550-7. DOI: 10.1016/j.vetmic.2013.07.004. View

Purcell B, Pruckler J, Clegg S . Nucleotide sequences of the genes encoding type 1 fimbrial subunits of Klebsiella pneumoniae and Salmonella typhimurium. J Bacteriol. 1987; 169(12):5831-4. PMC: 214169. DOI: 10.1128/jb.169.12.5831-5834.1987. View

Patterson S, Borewicz K, Johnson T, Xu W, Isaacson R . Characterization and differential gene expression between two phenotypic phase variants in Salmonella enterica serovar Typhimurium. PLoS One. 2012; 7(8):e43592. PMC: 3427373. DOI: 10.1371/journal.pone.0043592. View

Kuzminska-Bajor M, Kuczkowski M, Grzymajlo K, Wojciech L, Sabat M, Kisiela D . Decreased colonization of chicks by Salmonella enterica serovar Gallinarum expressing mannose-sensitive FimH adhesin from Salmonella enterica serovar Enteritidis. Vet Microbiol. 2012; 158(1-2):205-10. DOI: 10.1016/j.vetmic.2012.01.029. View

Grzymajlo K, Ugorski M, Suchanski J, Kedzierska A, Kolenda R, Jarzab A . The Novel Type 1 Fimbriae FimH Receptor Calreticulin Plays a Role in Host Specificity. Front Cell Infect Microbiol. 2017; 7:326. PMC: 5516122. DOI: 10.3389/fcimb.2017.00326. View

Owen S, Wenner N, Canals R, Makumi A, Hammarlof D, Gordon M . Characterization of the Prophage Repertoire of African Typhimurium ST313 Reveals High Levels of Spontaneous Induction of Novel Phage BTP1. Front Microbiol. 2017; 8:235. PMC: 5322425. DOI: 10.3389/fmicb.2017.00235. View

Nuccio S, Baumler A . Evolution of the chaperone/usher assembly pathway: fimbrial classification goes Greek. Microbiol Mol Biol Rev. 2007; 71(4):551-75. PMC: 2168650. DOI: 10.1128/MMBR.00014-07. View

Rajashekara G, Munir S, Alexeyev M, Halvorson D, Wells C, Nagaraja K . Pathogenic role of SEF14, SEF17, and SEF21 fimbriae in Salmonella enterica serovar enteritidis infection of chickens. Appl Environ Microbiol. 2000; 66(4):1759-63. PMC: 92059. DOI: 10.1128/AEM.66.4.1759-1763.2000. View

Hahn E, Wild P, Hermanns U, Sebbel P, Glockshuber R, Haner M . Exploring the 3D molecular architecture of Escherichia coli type 1 pili. J Mol Biol. 2002; 323(5):845-57. DOI: 10.1016/s0022-2836(02)01005-7. View

10.

Jacobson A, Lam L, Rajendram M, Tamburini F, Honeycutt J, Pham T . A Gut Commensal-Produced Metabolite Mediates Colonization Resistance to Salmonella Infection. Cell Host Microbe. 2018; 24(2):296-307.e7. PMC: 6223613. DOI: 10.1016/j.chom.2018.07.002. View

11.

Brosnahan A, Brown D . Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations. Vet Microbiol. 2011; 156(3-4):229-37. PMC: 3289732. DOI: 10.1016/j.vetmic.2011.10.017. View

12.

Wagner C, Hensel M . Adhesive mechanisms of Salmonella enterica. Adv Exp Med Biol. 2011; 715:17-34. DOI: 10.1007/978-94-007-0940-9_2. View

13.

Isaacson R, Argyilan C, Kwan L, Patterson S, Yoshinaga K . Phase variable switching of in vivo and environmental phenotypes of Salmonella typhimurium. Adv Exp Med Biol. 2000; 473:281-9. DOI: 10.1007/978-1-4615-4143-1_30. View

14.

Collazo C, Galan J . Requirement for exported proteins in secretion through the invasion-associated type III system of Salmonella typhimurium. Infect Immun. 1996; 64(9):3524-31. PMC: 174258. DOI: 10.1128/iai.64.9.3524-3531.1996. View

15.

McClelland M, Sanderson K, Spieth J, Clifton S, Latreille P, Courtney L . Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature. 2001; 413(6858):852-6. DOI: 10.1038/35101614. View

16.

Kuzminska-Bajor M, Grzymajlo K, Ugorski M . Type 1 fimbriae are important factors limiting the dissemination and colonization of mice by Salmonella Enteritidis and contribute to the induction of intestinal inflammation during Salmonella invasion. Front Microbiol. 2015; 6:276. PMC: 4391268. DOI: 10.3389/fmicb.2015.00276. View

17.

Kaniga K, Tucker S, Trollinger D, Galan J . Homologs of the Shigella IpaB and IpaC invasins are required for Salmonella typhimurium entry into cultured epithelial cells. J Bacteriol. 1995; 177(14):3965-71. PMC: 177125. DOI: 10.1128/jb.177.14.3965-3971.1995. View

18.

Martinez-Garcia E, de Lorenzo V . Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440. Environ Microbiol. 2011; 13(10):2702-16. DOI: 10.1111/j.1462-2920.2011.02538.x. View

19.

Humphries A, Raffatellu M, Winter S, Weening E, Kingsley R, Droleskey R . The use of flow cytometry to detect expression of subunits encoded by 11 Salmonella enterica serotype Typhimurium fimbrial operons. Mol Microbiol. 2003; 48(5):1357-76. DOI: 10.1046/j.1365-2958.2003.03507.x. View

20.

DUGUID J, Anderson E, Campbell I . Fimbriae and adhesive properties in Salmonellae. J Pathol Bacteriol. 1966; 92(1):107-38. DOI: 10.1002/path.1700920113. View