Toxicity Assessment of Reduced Graphene Oxide and Titanium Dioxide Nanomaterials on Gram-positive and Gram-negative Bacteria Under Normal Laboratory Lighting Condition

Overview

Journal Toxicol Rep

Date 2020 Jun 13

PMID 32528857

Citations 8

Authors

N S Ahmad

N Abdullah

F M Yasin

Affiliations

Soon will be listed here.

Abstract

Toxicity effect of reduced graphene oxide (rGO) and titanium dioxide (TiO) nanomaterials (NMs) on Gram-positive () and Gram-negative () bacteria was assessed. For both strains, study demonstrated that the toxicity was time and concentration dependent which led to reduction in growth rate and cell death. Upon NMs exposure, an instantaneous cell death in culture was observed. This is in contrast with , in which the culture growth remained in the log phase; however their growth rate constant, was reduced by ∼70%. The discrepancy between and was due to strain-specific response upon contact with NMs. TEM, SEM and EDX analysis revealed direct physical surface-surface interaction, as evidence from the adherence of NMs on the cell surface.

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References

Nair S, Sasidharan A, Rani V, Menon D, Nair S, Manzoor K . Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells. J Mater Sci Mater Med. 2008; 20 Suppl 1:S235-41. DOI: 10.1007/s10856-008-3548-5. View

Simon-Deckers A, Loo S, Mayne-LHermite M, Herlin-Boime N, Menguy N, Reynaud C . Size-, composition- and shape-dependent toxicological impact of metal oxide nanoparticles and carbon nanotubes toward bacteria. Environ Sci Technol. 2009; 43(21):8423-9. DOI: 10.1021/es9016975. View

Azam A, Ahmed A, Oves M, Khan M, Habib S, Memic A . Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a comparative study. Int J Nanomedicine. 2012; 7:6003-9. PMC: 3519005. DOI: 10.2147/IJN.S35347. View

Li C, Wang X, Chen F, Zhang C, Zhi X, Wang K . The antifungal activity of graphene oxide-silver nanocomposites. Biomaterials. 2013; 34(15):3882-90. DOI: 10.1016/j.biomaterials.2013.02.001. View

Gurr J, Wang A, Chen C, Jan K . Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells. Toxicology. 2005; 213(1-2):66-73. DOI: 10.1016/j.tox.2005.05.007. View

Raghupathi K, Koodali R, Manna A . Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles. Langmuir. 2011; 27(7):4020-8. DOI: 10.1021/la104825u. View

Feng Q, Wu J, Chen G, Cui F, Kim T, Kim J . A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res. 2000; 52(4):662-8. DOI: 10.1002/1097-4636(20001215)52:4<662::aid-jbm10>3.0.co;2-3. View

Ireland J, Klostermann P, Rice E, Clark R . Inactivation of Escherichia coli by titanium dioxide photocatalytic oxidation. Appl Environ Microbiol. 1993; 59(5):1668-70. PMC: 182135. DOI: 10.1128/aem.59.5.1668-1670.1993. View

Tu Y, Lv M, Xiu P, Huynh T, Zhang M, Castelli M . Destructive extraction of phospholipids from Escherichia coli membranes by graphene nanosheets. Nat Nanotechnol. 2013; 8(8):594-601. DOI: 10.1038/nnano.2013.125. View

10.

Azam A, Ahmed A, Oves M, Khan M, Memic A . Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and -negative bacterial strains. Int J Nanomedicine. 2012; 7:3527-35. PMC: 3405874. DOI: 10.2147/IJN.S29020. View

11.

Xiong D, Fang T, Yu L, Sima X, Zhu W . Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: acute toxicity, oxidative stress and oxidative damage. Sci Total Environ. 2011; 409(8):1444-52. DOI: 10.1016/j.scitotenv.2011.01.015. View

12.

Liu Y, Yu D, Zeng C, Miao Z, Dai L . Biocompatible graphene oxide-based glucose biosensors. Langmuir. 2010; 26(9):6158-60. DOI: 10.1021/la100886x. View

13.

Liu S, Zeng T, Hofmann M, Burcombe E, Wei J, Jiang R . Antibacterial activity of graphite, graphite oxide, graphene oxide, and reduced graphene oxide: membrane and oxidative stress. ACS Nano. 2011; 5(9):6971-80. DOI: 10.1021/nn202451x. View

14.

Jahan S, Yusoff I, Alias Y, Abu Bakar A . Reviews of the toxicity behavior of five potential engineered nanomaterials (ENMs) into the aquatic ecosystem. Toxicol Rep. 2017; 4:211-220. PMC: 5615119. DOI: 10.1016/j.toxrep.2017.04.001. View

15.

Jones N, Ray B, Ranjit K, Manna A . Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms. FEMS Microbiol Lett. 2007; 279(1):71-6. DOI: 10.1111/j.1574-6968.2007.01012.x. View

16.

Kim J, Kuk E, Yu K, Kim J, Park S, Lee H . Antimicrobial effects of silver nanoparticles. Nanomedicine. 2007; 3(1):95-101. DOI: 10.1016/j.nano.2006.12.001. View

17.

Takenaka S, Karg E, Roth C, Schulz H, Ziesenis A, Heinzmann U . Pulmonary and systemic distribution of inhaled ultrafine silver particles in rats. Environ Health Perspect. 2001; 109 Suppl 4:547-51. PMC: 1240579. DOI: 10.1289/ehp.01109s4547. View

18.

Zhou Y, Kong Y, Kundu S, Cirillo J, Liang H . Antibacterial activities of gold and silver nanoparticles against Escherichia coli and bacillus Calmette-Guérin. J Nanobiotechnology. 2012; 10:19. PMC: 3405418. DOI: 10.1186/1477-3155-10-19. View

19.

Das M, Sarma R, Saikia R, Kale V, Shelke M, Sengupta P . Synthesis of silver nanoparticles in an aqueous suspension of graphene oxide sheets and its antimicrobial activity. Colloids Surf B Biointerfaces. 2010; 83(1):16-22. DOI: 10.1016/j.colsurfb.2010.10.033. View

20.

Kasemets K, Ivask A, Dubourguier H, Kahru A . Toxicity of nanoparticles of ZnO, CuO and TiO2 to yeast Saccharomyces cerevisiae. Toxicol In Vitro. 2009; 23(6):1116-22. DOI: 10.1016/j.tiv.2009.05.015. View