Biophysical Parameters of GEVIs: Considerations for Imaging Voltage

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2020 Jun 11

PMID 32521239

Citations 7

Authors

Jun Kyu Rhee

Lee Min Leong

Md Sofequl Islam Mukim

Bok Eum Kang

Sungmoo Lee

Laura Bilbao-Broch

Bradley J Baker

Affiliations

Soon will be listed here.

Abstract

Genetically encoded voltage indicators (GEVIs) continue to evolve, resulting in many different probes with varying strengths and weaknesses. Developers of new GEVIs tend to highlight their positive features. A recent article from an independent laboratory has compared the signal/noise ratios of a number of GEVIs. Such a comparison can be helpful to investigators eager to try to image the voltage of excitable cells. In this perspective, we will present examples of how the biophysical features of GEVIs affect the imaging of excitable cells in an effort to assist researchers when considering probes for their specific needs.

Citing Articles

A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic events.

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An Ultrasensitive Genetically Encoded Voltage Indicator Uncovers the Electrical Activity of Non-Excitable Cells.

Ruhl P, Nair A, Gawande N, Dehiwalage S, Munster L, Schonherr R Adv Sci (Weinh). 2024; 11(20):e2307938.

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Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.

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Single Photon Kilohertz Frame Rate Imaging of Neural Activity.

Tian T, Yuan Y, Mitra S, Gyongy I, Nolan M Adv Sci (Weinh). 2022; 9(31):e2203018.

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Conserved Amino Acids Residing Outside the Voltage Field Can Shift the Voltage Sensitivity and Increase the Signal Speed and Size of Based GEVIs.

Sepehri Rad M, Cohen L, Baker B Front Cell Dev Biol. 2022; 10:868143.

PMID: 35784472 PMC: 9243531. DOI: 10.3389/fcell.2022.868143.

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