Untargeted Lipidomics Using Liquid Chromatography-ion Mobility-mass Spectrometry Reveals Novel Triacylglycerides in Human Milk

Overview

Journal Sci Rep

Specialty Science

Date 2020 Jun 11

PMID 32518313

Citations 17

Authors

Alexandra D George

Melvin C L Gay

Mary E Wlodek

Robert D Trengove

Kevin Murray

Donna T Geddes

Affiliations

Soon will be listed here.

Abstract

Human milk provides the infant with the essential nutritive and non-nutritive factors required for health, growth and development. The human milk lipidome is complex, but comprises predominantly triacylglycerides. Historically, the fatty acid profile of the entire human milk lipidome has been investigated, and many relationships have been identified between infant health and fatty acids. Most of these fatty acids are, however, delivered to the infant as triacylglycerides. Using liquid chromatography-ion mobility-mass spectrometry, the objective of this study was to characterise the triacylglyceride profile of human milk and elucidate relationships between the triacylglyceride profile and infant outcomes in a cohort of 10 exclusively breastfeeding woman-infant dyads. 205 triacylglycerides were identified, including 98 previously not reported in human milk. The dose of specific triacylglycerides differed in relation to infant health, such as lauric acid containing TAGs, which were delivered in significantly higher dose to healthy infants compared to unwell infants.

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