A Testis-Derived Hydrogel As an Efficient Feeder-Free Culture Platform to Promote Mouse Spermatogonial Stem Cell Proliferation and Differentiation

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2020 Jun 9

PMID 32509769

Citations 17

Authors

Yan Yang

Qilian Lin

Chengxing Zhou

Quan Li

Ziyi Li

Zhen Cao

Jinlian Liang

Hanhao Li

Jiaxin Mei

Qihao Zhang

Qi Xiang

Wei Xue

Yadong Huang

Affiliations

Soon will be listed here.

Abstract

Fertility preservation and assisted reproductive medicine require effective culture systems for the successful proliferation and differentiation of spermatogonial stem cells (SSCs). Many SSC culture systems require the addition of feeder cells at each subculture, which is tedious and inefficient. Here, we prepared decellularized testicular matrix (DTM) from testicular tissue, which preserved essential structural proteins of testis. The DTM was then solubilized and induced to form a porous hydrogel scaffold with randomly oriented fibrillar structures that exhibited good cytocompatibility. The viability of SSCs inoculated onto DTM hydrogel scaffolds was significantly higher than those inoculated on Matrigel or laminin, and intracellular gene expression and DNA imprinting patterns were similar to that of native SSCs. Additionally, DTM promoted SSC differentiation into round spermatids. More importantly, the DTM hydrogel supported SSC proliferation and differentiation without requiring additional somatic cells. The DTM hydrogel scaffold culture system provided an alternative and simple method for culturing SSCs that eliminates potential variability and contamination caused by feeder cells. It might be a valuable tool for reproductive medicine.

Citing Articles

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