Effects of Short- and Long-term Glucocorticoid-induced Osteoporosis on Plasma Metabolome and Lipidome of Ovariectomized Sheep

Overview

Journal BMC Musculoskelet Disord

Publisher Biomed Central

Specialties Orthopedics
Physiology

Date 2020 Jun 7

PMID 32503480

Citations 11

Authors

Diana Cabrera

Marlena Kruger

Frances M Wolber

Nicole C Roy

Karl Fraser

Affiliations

Soon will be listed here.

Abstract

Background: Understanding the metabolic and lipidomic changes that accompany bone loss in osteoporosis might provide insights about the mechanisms behind molecular changes and facilitate developing new drugs or nutritional strategies for osteoporosis prevention. This study aimed to examine the effects of short- or long-term glucocorticoid-induced osteoporosis on plasma metabolites and lipids of ovariectomized (OVX) sheep.

Methods: Twenty-eight aged ewes were divided randomly into four groups: an OVX group, OVX in combination with glucocorticoids for two months (OVXG2), and OVX in combination with five doses of glucocorticoids (OVXG5) to induce bone loss, and a control group. Liquid chromatography-mass spectrometry untargeted metabolomic analysis was applied to monthly plasma samples to follow the progression of osteoporosis over five months.

Results: The metabolite profiles revealed significant differences in the plasma metabolome of OVX sheep and OVXG when compared with the control group by univariate analysis. Nine metabolites were altered, namely 5-methoxytryptophan, valine, methionine, tryptophan, glutaric acid, 2-pyrrolidone-5-carboxylic acid, indole-3-carboxaldehyde, 5-hydroxylysine and malic acid. Similarly, fifteen lipids were perturbed from multiple lipid classes such as lysophoslipids, phospholipids and ceramides.

Conclusion: This study showed that OVX and glucocorticoid interventions altered the metabolite and lipid profiles of sheep, suggesting that amino acid and lipid metabolisms are potentially the main perturbed metabolic pathways regulating bone loss in OVX sheep.

Citing Articles

The association between metabolite profiles and impaired bone microstructure in adult growth hormone deficient rats.

Guo X, Liu S, Hu W, Lyu X, Xu H, Zhu H BMC Musculoskelet Disord. 2024; 25(1):883.

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The critical role of toll-like receptor 4 in bone remodeling of osteoporosis: from inflammation recognition to immunity.

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Linking the relation between gut microbiota and glucocorticoid-induced osteoporosis.

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Application of metabolomics in osteoporosis research.

Zhao Z, Cai Z, Chen A, Cai M, Yang K Front Endocrinol (Lausanne). 2022; 13:993253.

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Lipidomics Profiling of Patients with Low Bone Mineral Density (LBMD).

Aleidi S, Al-Ansari M, Alnehmi E, Malkawi A, Alodaib A, Alshaker M Int J Mol Sci. 2022; 23(19).

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