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ESHRE PGT Consortium Good Practice Recommendations for the Detection of Monogenic Disorders

Overview
Journal Hum Reprod Open
Publisher Oxford University Press
Specialty Reproductive Medicine
Date 2020 Jun 6
PMID 32500103
Citations 50
Authors
Filipa Carvalho
Celine Moutou
Eftychia Dimitriadou
Jos Dreesen
Carles Gimenez
Veerle Goossens
Georgia Kakourou
Nathalie Vermeulen
Daniela Zuccarello
Martine De Rycke
Affiliations
Soon will be listed here.
Abstract

The field of preimplantation genetic testing (PGT) is evolving fast and best practice advice is essential for regulation and standardisation of diagnostic testing. The previous ESHRE guidelines on best practice for PGD, published in 2005 and 2011, are considered outdated, and the development of new papers outlining recommendations for good practice in PGT was necessary. The current paper provides recommendations on the technical aspects of PGT for monogenic/single-gene defects (PGT-M) and covers recommendations on basic methods for PGT-M and testing strategies. Furthermore, some specific recommendations are formulated for special cases, including pathogenic variants, consanguineous couples, HLA typing, exclusion testing and disorders caused by pathogenic variants in the mitochondrial DNA. This paper is one of a series of four papers on good practice recommendations on PGT. The other papers cover the organisation of a PGT centre, embryo biopsy and tubing and the technical aspects of PGT for chromosomal structural rearrangements/aneuploidies. Together, these papers should assist scientists interested in PGT in developing the best laboratory and clinical practice possible.

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