Silicon-induced Thermotolerance in Solanum Lycopersicum L. Via Activation of Antioxidant System, Heat Shock Proteins, and Endogenous Phytohormones

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2020 Jun 5

PMID 32493420

Citations 19

Authors

Adil Khan

Abdul Latif Khan

Muhammad Imran

Sajjad Asaf

Yoon-Ha Kim

Saqib Bilal

Muhammad Numan

Ahmed Al-Harrasi

Ahmed Al-Rawahi

In-Jung Lee

Affiliations

Soon will be listed here.

Abstract

Background: Abiotic stresses (e.g., heat or limited water and nutrient availability) limit crop production worldwide. With the progression of climate change, the severity and variation of these stresses are expected to increase. Exogenous silicon (Si) has shown beneficial effects on plant growth; however, its role in combating the negative effects of heat stress and their underlying molecular dynamics are not fully understood.

Results: Exogenous Si significantly mitigated the adverse impact of heat stress by improving tomato plant biomass, photosynthetic pigments, and relative water content. Si induced stress tolerance by decreasing the concentrations of superoxide anions and malondialdehyde, as well as mitigating oxidative stress by increasing the gene expression for antioxidant enzymes (peroxidases, catalases, ascorbate peroxidases, superoxide dismutases, and glutathione reductases) under stress conditions. This was attributed to increased Si uptake in the shoots via the upregulation of low silicon (SlLsi1 and SlLsi2) gene expression under heat stress. Interestingly, Si stimulated the expression and transcript accumulation of heat shock proteins by upregulating heat transcription factors (Hsfs) such as SlHsfA1a-b, SlHsfA2-A3, and SlHsfA7 in tomato plants under heat stress. On the other hand, defense and stress signaling-related endogenous phytohormones (salicylic acid [SA]/abscisic acid [ABA]) exhibited a decrease in their concentration and biosynthesis following Si application. Additionally, the mRNA and gene expression levels for SA (SlR1b1, SlPR-P2, SlICS, and SlPAL) and ABA (SlNCEDI) were downregulated after exposure to stress conditions.

Conclusion: Si treatment resulted in greater tolerance to abiotic stress conditions, exhibiting higher plant growth dynamics and molecular physiology by regulating the antioxidant defense system, SA/ABA signaling, and Hsfs during heat stress.

Citing Articles

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References

Shahzad R, Waqas M, Khan A, Asaf S, Khan M, Kang S . Seed-borne endophytic Bacillus amyloliquefaciens RWL-1 produces gibberellins and regulates endogenous phytohormones of Oryza sativa. Plant Physiol Biochem. 2016; 106:236-43. DOI: 10.1016/j.plaphy.2016.05.006. View

Liu L, Han R, Yu N, Zhang W, Xing L, Xie D . A method for extracting high-quality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense. PLoS One. 2018; 13(5):e0196592. PMC: 5929529. DOI: 10.1371/journal.pone.0196592. View

Kidd P, Llugany M, Poschenrieder C, Gunse B, Barcelo J . The role of root exudates in aluminium resistance and silicon-induced amelioration of aluminium toxicity in three varieties of maize (Zea mays L.). J Exp Bot. 2001; 52(359):1339-52. View

Wang L, Fan L, Loescher W, Duan W, Liu G, Cheng J . Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves. BMC Plant Biol. 2010; 10:34. PMC: 2848757. DOI: 10.1186/1471-2229-10-34. View

Shi Y, Zhang Y, Han W, Feng R, Hu Y, Guo J . Silicon Enhances Water Stress Tolerance by Improving Root Hydraulic Conductance in Solanum lycopersicum L. Front Plant Sci. 2016; 7:196. PMC: 4761792. DOI: 10.3389/fpls.2016.00196. View

Mishra N, Tuteja R, Tuteja N . Signaling through MAP kinase networks in plants. Arch Biochem Biophys. 2006; 452(1):55-68. DOI: 10.1016/j.abb.2006.05.001. View

Shinozaki K, Yamaguchi-Shinozaki K, Seki M . Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol. 2003; 6(5):410-7. DOI: 10.1016/s1369-5266(03)00092-x. View

AEBI H . Catalase in vitro. Methods Enzymol. 1984; 105:121-6. DOI: 10.1016/s0076-6879(84)05016-3. View

Xia X, Zhou Y, Shi K, Zhou J, Foyer C, Yu J . Interplay between reactive oxygen species and hormones in the control of plant development and stress tolerance. J Exp Bot. 2015; 66(10):2839-56. DOI: 10.1093/jxb/erv089. View

10.

Zhang F, Wang Y, Lou Z, Dong J . Effect of heavy metal stress on antioxidative enzymes and lipid peroxidation in leaves and roots of two mangrove plant seedlings (Kandelia candel and Bruguiera gymnorrhiza). Chemosphere. 2006; 67(1):44-50. DOI: 10.1016/j.chemosphere.2006.10.007. View

11.

Agarwal P, Agarwal P, Reddy M, Sopory S . Role of DREB transcription factors in abiotic and biotic stress tolerance in plants. Plant Cell Rep. 2006; 25(12):1263-74. DOI: 10.1007/s00299-006-0204-8. View

12.

Mittler R, Blumwald E . Genetic engineering for modern agriculture: challenges and perspectives. Annu Rev Plant Biol. 2010; 61:443-62. DOI: 10.1146/annurev-arplant-042809-112116. View

13.

Kar M, Mishra D . Catalase, Peroxidase, and Polyphenoloxidase Activities during Rice Leaf Senescence. Plant Physiol. 1976; 57(2):315-9. PMC: 542015. DOI: 10.1104/pp.57.2.315. View

14.

Zarate S, Kempema L, Walling L . Silverleaf whitefly induces salicylic acid defenses and suppresses effectual jasmonic acid defenses. Plant Physiol. 2006; 143(2):866-75. PMC: 1803729. DOI: 10.1104/pp.106.090035. View

15.

Kim Y, Khan A, Kim D, Lee S, Kim K, Waqas M . Silicon mitigates heavy metal stress by regulating P-type heavy metal ATPases, Oryza sativa low silicon genes, and endogenous phytohormones. BMC Plant Biol. 2014; 14:13. PMC: 3893592. DOI: 10.1186/1471-2229-14-13. View

16.

Bharti K, von Koskull-Doring P, Bharti S, Kumar P, Tintschl-Korbitzer A, Treuter E . Tomato heat stress transcription factor HsfB1 represents a novel type of general transcription coactivator with a histone-like motif interacting with the plant CREB binding protein ortholog HAC1. Plant Cell. 2004; 16(6):1521-35. PMC: 490043. DOI: 10.1105/tpc.019927. View

17.

Li X, Wei J, Scott E, Liu J, Guo S, Li Y . Exogenous Melatonin Alleviates Cold Stress by Promoting Antioxidant Defense and Redox Homeostasis in Camellia sinensis L. Molecules. 2018; 23(1). PMC: 6017414. DOI: 10.3390/molecules23010165. View

18.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

19.

Huang F, Wen X, Cai Y, Cai K . Silicon-Mediated Enhancement of Heavy Metal Tolerance in Rice at Different Growth Stages. Int J Environ Res Public Health. 2018; 15(10). PMC: 6210271. DOI: 10.3390/ijerph15102193. View

20.

Zhou R, Yu X, Ottosen C, Rosenqvist E, Zhao L, Wang Y . Drought stress had a predominant effect over heat stress on three tomato cultivars subjected to combined stress. BMC Plant Biol. 2017; 17(1):24. PMC: 5264292. DOI: 10.1186/s12870-017-0974-x. View