Stress-induced Phosphorylation of CLIP-170 by JNK Promotes Microtubule Rescue

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2020 Jun 4

PMID 32491151

Citations 6

Authors

Helene Henrie

Dalal Bakhos-Douaihy

Isabelle Cantaloube

Antoine Pilon

Maya Talantikite

Virginie Stoppin-Mellet

Anita Baillet

Christian Pous

Beatrice Benoit

Affiliations

Soon will be listed here.

Abstract

The stress-induced c-Jun N-terminal kinase (JNK) controls microtubule dynamics by enhancing both microtubule growth and rescues. Here, we show that upon cell stress, JNK directly phosphorylates the microtubule rescue factor CLIP-170 in its microtubule-binding domain to increase its rescue-promoting activity. Phosphomimetic versions of CLIP-170 enhance its ability to promote rescue events in vitro and in cells. Furthermore, while phosphomimetic mutations do not alter CLIP-170's capability to form comets at growing microtubule ends, both phosphomimetic mutations and JNK activation increase the occurrence of CLIP-170 remnants on the microtubule lattice at the rear of comets. As the CLIP-170 remnants, which are potential sites of microtubule rescue, display a shorter lifetime when CLIP-170 is phosphorylated, we propose that instead of acting at the time of rescue occurrence, CLIP-170 would rather contribute in preparing the microtubule lattice for future rescues at these predetermined sites.

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