» Articles » PMID: 32491151

Stress-induced Phosphorylation of CLIP-170 by JNK Promotes Microtubule Rescue

Overview
Journal J Cell Biol
Specialty Cell Biology
Date 2020 Jun 4
PMID 32491151
Citations 6
Authors
Affiliations
Soon will be listed here.
Abstract

The stress-induced c-Jun N-terminal kinase (JNK) controls microtubule dynamics by enhancing both microtubule growth and rescues. Here, we show that upon cell stress, JNK directly phosphorylates the microtubule rescue factor CLIP-170 in its microtubule-binding domain to increase its rescue-promoting activity. Phosphomimetic versions of CLIP-170 enhance its ability to promote rescue events in vitro and in cells. Furthermore, while phosphomimetic mutations do not alter CLIP-170's capability to form comets at growing microtubule ends, both phosphomimetic mutations and JNK activation increase the occurrence of CLIP-170 remnants on the microtubule lattice at the rear of comets. As the CLIP-170 remnants, which are potential sites of microtubule rescue, display a shorter lifetime when CLIP-170 is phosphorylated, we propose that instead of acting at the time of rescue occurrence, CLIP-170 would rather contribute in preparing the microtubule lattice for future rescues at these predetermined sites.

Citing Articles

Phase separation of +TIP networks regulates microtubule dynamics.

Miesch J, Wimbish R, Velluz M, Aumeier C Proc Natl Acad Sci U S A. 2023; 120(35):e2301457120.

PMID: 37603768 PMC: 10469336. DOI: 10.1073/pnas.2301457120.


The autophagy inducer SMER28 attenuates microtubule dynamics mediating neuroprotection.

Kirchenwitz M, Stahnke S, Grunau K, Melcher L, van Ham M, Rottner K Sci Rep. 2022; 12(1):17805.

PMID: 36284196 PMC: 9596692. DOI: 10.1038/s41598-022-20563-3.


Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration.

Hu Y, Xie Q, Wu X, Liu W, Li D, Li C Cell Death Dis. 2022; 13(10):856.

PMID: 36209218 PMC: 9547975. DOI: 10.1038/s41419-022-05306-6.


A kinesin-1 variant reveals motor-induced microtubule damage in cells.

Budaitis B, Badieyan S, Yue Y, Blasius T, Reinemann D, Lang M Curr Biol. 2022; 32(11):2416-2429.e6.

PMID: 35504282 PMC: 9993403. DOI: 10.1016/j.cub.2022.04.020.


Cytoskeleton and Associated Proteins: Pleiotropic JNK Substrates and Regulators.

Benoit B, Baillet A, Pous C Int J Mol Sci. 2021; 22(16).

PMID: 34445080 PMC: 8395060. DOI: 10.3390/ijms22168375.


References
1.
Bogoyevitch M, Kobe B . Uses for JNK: the many and varied substrates of the c-Jun N-terminal kinases. Microbiol Mol Biol Rev. 2006; 70(4):1061-95. PMC: 1698509. DOI: 10.1128/MMBR.00025-06. View

2.
Lawrence E, Arpag G, Norris S, Zanic M . Human CLASP2 specifically regulates microtubule catastrophe and rescue. Mol Biol Cell. 2018; 29(10):1168-1177. PMC: 5935067. DOI: 10.1091/mbc.E18-01-0016. View

3.
Lansbergen G, Komarova Y, Modesti M, Wyman C, Hoogenraad C, Goodson H . Conformational changes in CLIP-170 regulate its binding to microtubules and dynactin localization. J Cell Biol. 2004; 166(7):1003-14. PMC: 2172020. DOI: 10.1083/jcb.200402082. View

4.
Pierre P, Scheel J, Rickard J, Kreis T . CLIP-170 links endocytic vesicles to microtubules. Cell. 1992; 70(6):887-900. DOI: 10.1016/0092-8674(92)90240-d. View

5.
Arnal I, Heichette C, Diamantopoulos G, Chretien D . CLIP-170/tubulin-curved oligomers coassemble at microtubule ends and promote rescues. Curr Biol. 2004; 14(23):2086-95. DOI: 10.1016/j.cub.2004.11.055. View