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Use of a Free Viral Immunofluorescence Assay to Detect Human Reovirus-like Agent in Human Stools

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Journal Infect Immun
Date 1977 May 1
PMID 324909
Citations 13
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Abstract

Human reovirus-like agent (HRVLA) is a major cause of gastroenteritis in infants and young children in many parts of the world. Detection of HRVLA in stools is impractical with the techniques currently available. We describe a rapid immunofluorescence assay for detection of HRVLA in stools. Results with this assay agreed well with results obtained from examination of stool specimens by electron microscopy.

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References
1.
Mebus C, Wyatt R, Sharpee R, Sereno M, Kalica A, Kapikian A . Diarrhea in gnotobiotic calves caused by the reovirus-like agent of human infantile gastroenteritis. Infect Immun. 1976; 14(2):471-4. PMC: 420908. DOI: 10.1128/iai.14.2.471-474.1976. View

2.
Kapikian A, Kim H, Wyatt R, Cline W, PARROTT R, Chanock R . Recent advances in the aetiology of viral gastroenteritis. Ciba Found Symp. 1976; (42):273-309. DOI: 10.1002/9780470720240.ch16. View

3.
Kapikian A, Wyatt R, Dolin R, Thornhill T, Kalica A, Chanock R . Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol. 1972; 10(5):1075-81. PMC: 356579. DOI: 10.1128/JVI.10.5.1075-1081.1972. View

4.
Almeida J, WATERSON A . The morphology of virus-antibody interaction. Adv Virus Res. 1969; 15:307-38. PMC: 7173053. DOI: 10.1016/s0065-3527(08)60878-7. View

5.
Davidson G, Bishop R, TOWNLEY R, HOLMES I . Importance of a new virus in acute sporadic enteritis in children. Lancet. 1975; 1(7901):242-6. DOI: 10.1016/s0140-6736(75)91140-x. View