A Nutritional Supplement (DìLsh) Improves the Inflammatory Cytokines Response, Oxidative Stress Markers and Clinical Signs in Dogs Naturally Infected by

Overview

Journal Animals (Basel)

Date 2020 Jun 4

PMID 32485886

Citations 3

Authors

Vincenzo Mastellone

Nadia Musco

Giuseppe Vassalotti

Diego Piantedosi

Alessandro Vastolo

Monica Isabella Cutrignelli

Domenico Britti

Laura Cortese

Pietro Lombardi

Affiliations

Soon will be listed here.

Abstract

The possibility to associate nutraceuticals, as immune-modulating tools, to the treatment of visceral leishmaniosis is a matter of great interest. In this study, we investigated whether the administration of a nutritional supplement (DìLsh, Dynamopet SRL, Verona, Italy) was able to exert beneficial effects on the inflammatory state and oxidative stress of the dogs naturally infected by Leishmania infantum. To this purpose, specific parameters, namely Tumor Necrosis Factor -alpha (TNF), Interleukin-6 (IL-6), Inteleukin-10 (IL-10), leptin, derivates of Reactive Oxigen Metabolites (d-Roms) and Biological Antioxidant Potential (BAP), as well as the haematological and biochemical profiles of the infected dogs, were investigated upon the treatment with the nutritional supplement and compared with the conventional pharmacological anti- therapy. The animals underwent complete clinical examination and blood sample collection before (T0) and 3 months after (T90) the onset of the two treatments. The two treatments showed similar results: significant clinical improvement, ELISA positivity and IgG decrease, an increase in IL-10, and a decrease in IL-6 were observed in animals treated with the nutritional supplement. A decrease in d-Roms and an increase in BAP were also detected in both groups. On the whole, the nutritional supplement possesses anti-inflammatory and antioxidant properties, suggesting that it may support animals' health and be useful to extend the time a drug therapy is needed.

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References

Corpas-Lopez V, Merino-Espinosa G, Acedo-Sanchez C, Diaz-Saez V, Navarro-Moll M, Morillas-Marquez F . Effectiveness of the sesquiterpene (-)-α-bisabolol in dogs with naturally acquired canine leishmaniosis: an exploratory clinical trial. Vet Res Commun. 2018; 42(2):121-130. DOI: 10.1007/s11259-018-9714-4. View

Nestel P, Clifton P, Colquhoun D, Noakes M, Mori T, Sullivan D . Indications for Omega-3 Long Chain Polyunsaturated Fatty Acid in the Prevention and Treatment of Cardiovascular Disease. Heart Lung Circ. 2015; 24(8):769-79. DOI: 10.1016/j.hlc.2015.03.020. View

Di Loria A, Squillacioti C, De Luca A, Veneziano V, Mirabella N, Guccione J . Increased leptin mRNA expression in the blood of dogs naturally infected by Leishmania infantum. Vet J. 2014; 202(3):634-6. DOI: 10.1016/j.tvjl.2014.10.010. View

Zhou X, Seto S, Chang D, Kiat H, Razmovski-Naumovski V, Chan K . Synergistic Effects of Chinese Herbal Medicine: A Comprehensive Review of Methodology and Current Research. Front Pharmacol. 2016; 7:201. PMC: 4940614. DOI: 10.3389/fphar.2016.00201. View

Abella V, Scotece M, Conde J, Pino J, Gonzalez-Gay M, Gomez-Reino J . Leptin in the interplay of inflammation, metabolism and immune system disorders. Nat Rev Rheumatol. 2017; 13(2):100-109. DOI: 10.1038/nrrheum.2016.209. View

Wagner H, Ulrich-Merzenich G . Synergy research: approaching a new generation of phytopharmaceuticals. Phytomedicine. 2009; 16(2-3):97-110. DOI: 10.1016/j.phymed.2008.12.018. View

Roura X, Fondati A, Lubas G, Gradoni L, Maroli M, Oliva G . Prognosis and monitoring of leishmaniasis in dogs: a working group report. Vet J. 2013; 198(1):43-7. DOI: 10.1016/j.tvjl.2013.04.001. View

Procaccini C, Pucino V, De Rosa V, Marone G, Matarese G . Neuro-endocrine networks controlling immune system in health and disease. Front Immunol. 2014; 5:143. PMC: 3985001. DOI: 10.3389/fimmu.2014.00143. View

Perez-Perez A, Vilarino-Garcia T, Fernandez-Riejos P, Martin-Gonzalez J, Segura-Egea J, Sanchez-Margalet V . Role of leptin as a link between metabolism and the immune system. Cytokine Growth Factor Rev. 2017; 35:71-84. DOI: 10.1016/j.cytogfr.2017.03.001. View

10.

Kuo Y, Tsai W, Wang J, Chang S, Lin C, Shiao M . Regulation of bronchoalveolar lavage fluids cell function by the immunomodulatory agents from Cordyceps sinensis. Life Sci. 2001; 68(9):1067-82. DOI: 10.1016/s0024-3205(00)01011-0. View

11.

Mustafa A, Caprioli G, Ricciutelli M, Maggi F, Marin R, Vittori S . Comparative HPLC/ESI-MS and HPLC/DAD study of different populations of cultivated, wild and commercial Gentiana lutea L. Food Chem. 2014; 174:426-33. DOI: 10.1016/j.foodchem.2014.11.089. View

12.

Chiou W, Chang P, Chou C, Chen C . Protein constituent contributes to the hypotensive and vasorelaxant activities of Cordyceps sinensis. Life Sci. 2001; 66(14):1369-76. DOI: 10.1016/s0024-3205(00)00445-8. View

13.

Benzie I, Strain J . The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996; 239(1):70-6. DOI: 10.1006/abio.1996.0292. View

14.

Sakaguchi S . The origin of FOXP3-expressing CD4+ regulatory T cells: thymus or periphery. J Clin Invest. 2003; 112(9):1310-2. PMC: 228490. DOI: 10.1172/JCI20274. View

15.

Cortese L, Annunziatella M, Palatucci A, Rubino V, Piantedosi D, Di Loria A . Regulatory T cells, Cytotoxic T lymphocytes and a T(H)1 cytokine profile in dogs naturally infected by Leishmania infantum. Res Vet Sci. 2013; 95(3):942-9. DOI: 10.1016/j.rvsc.2013.08.005. View

16.

Lombardi P, Palatucci A, Giovazzino A, Mastellone V, Ruggiero G, Rubino V . Clinical and Immunological Response in Dogs Naturally Infected by Treated with a Nutritional Supplement. Animals (Basel). 2019; 9(8). PMC: 6721201. DOI: 10.3390/ani9080501. View

17.

Khoo C, Cunnick J, Friesen K, Gross K, Wedekind K, Jewell D . The role of supplementary dietary antioxidants on immune response in puppies. Vet Ther. 2005; 6(1):43-56. View

18.

Sudheendran S, Chang C, Deckelbaum R . N-3 vs. saturated fatty acids: effects on the arterial wall. Prostaglandins Leukot Essent Fatty Acids. 2010; 82(4-6):205-9. PMC: 2878127. DOI: 10.1016/j.plefa.2010.02.020. View

19.

Marsella R, Santoro D, Ahrens K . Early exposure to probiotics in a canine model of atopic dermatitis has long-term clinical and immunological effects. Vet Immunol Immunopathol. 2012; 146(2):185-9. DOI: 10.1016/j.vetimm.2012.02.013. View

20.

Adalid-Peralta L, Fragoso G, Fleury A, Sciutto E . Mechanisms underlying the induction of regulatory T cells and its relevance in the adaptive immune response in parasitic infections. Int J Biol Sci. 2011; 7(9):1412-26. PMC: 3221948. DOI: 10.7150/ijbs.7.1412. View