Imaging Flow Cytometry Analysis of CEACAM Binding to Opa-Expressing Neisseria Gonorrhoeae

Overview

Journal Cytometry A

Specialties Cell Biology
Radiology

Date 2020 Jun 3

PMID 32484607

Citations 8

Authors

Lacie M Werner

Allison Palmer

Asya Smirnov

Meagan Belcher Dufrisne

Linda Columbus

Alison K Criss

Affiliations

Soon will be listed here.

Abstract

Human carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) are a family of receptors that mediate intercellular interactions. Pathogenic bacteria have ligands that bind CEACAMs on human cells. Neisseria gonorrhoeae (Gc) encodes numerous unique outer membrane opacity-associated (Opa) proteins that are ligands for one or more CEACAMs. CEACAMs that are expressed on epithelial cells facilitate Gc colonization, while those expressed on neutrophils affect phagocytosis and consequent intracellular survival of Gc. Since Opa protein expression is phase-variable, variations in receptor tropism affect how individual bacteria within a population interact with host cells. Here we report the development of a rapid, quantitative method for collecting and analyzing fluorescence intensity data from thousands of cells in a population using imaging flow cytometry to detect N-CEACAM bound to the surface of Opa-expressing Gc. We use this method to confirm previous findings regarding Opa-CEACAM interactions and to examine the receptor-ligand interactions of Gc expressing other Opa proteins, as well as for other N-CEACAM proteins. © 2020 International Society for Advancement of Cytometry.

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