NADPH Oxidases: Current Aspects and Tools

Overview

Journal Redox Biol

Specialties Biology
Cell Biology
Endocrinology

Date 2020 Jun 2

PMID 32480354

Citations 37

Authors

Katrin Schroder

Affiliations

Soon will be listed here.

Abstract

Reactive oxygen species (ROS) have been shown or at least suggested to play an essential role for cellular signaling as second messengers. NADPH oxidases represent a source of controlled ROS formation. Accordingly, understanding the role of individual NADPH oxidases bears potential to interfere with intracellular signaling cascades without disturbing the signaling itself. Many tools have been developed to study or inhibit the functions and roles of the NADPH oxidases. This short review summarizes diseases, potentially associated with NADPH oxidases, genetically modified animals, and inhibitors.

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References

Thao-Vi Dao V, Elbatreek M, Altenhofer S, Casas A, Pachado M, Neullens C . Isoform-selective NADPH oxidase inhibitor panel for pharmacological target validation. Free Radic Biol Med. 2019; 148:60-69. DOI: 10.1016/j.freeradbiomed.2019.12.038. View

Olofsson P, Holmberg J, Tordsson J, Lu S, Akerstrom B, Holmdahl R . Positional identification of Ncf1 as a gene that regulates arthritis severity in rats. Nat Genet. 2002; 33(1):25-32. DOI: 10.1038/ng1058. View

Joo J, Oh H, Kim M, An E, Kim R, Lee S . NADPH Oxidase 1 Activity and ROS Generation Are Regulated by Grb2/Cbl-Mediated Proteasomal Degradation of NoxO1 in Colon Cancer Cells. Cancer Res. 2016; 76(4):855-65. DOI: 10.1158/0008-5472.CAN-15-1512. View

Schroder K, Kohnen A, Aicher A, Liehn E, Buchse T, Stein S . NADPH oxidase Nox2 is required for hypoxia-induced mobilization of endothelial progenitor cells. Circ Res. 2009; 105(6):537-44. DOI: 10.1161/CIRCRESAHA.109.205138. View

Helfinger V, Palfi K, Weigert A, Schroder K . The NADPH Oxidase Nox4 Controls Macrophage Polarization in an NFB-Dependent Manner. Oxid Med Cell Longev. 2019; 2019:3264858. PMC: 6501210. DOI: 10.1155/2019/3264858. View

Freyhaus H, Huntgeburth M, Wingler K, Schnitker J, Baumer A, Vantler M . Novel Nox inhibitor VAS2870 attenuates PDGF-dependent smooth muscle cell chemotaxis, but not proliferation. Cardiovasc Res. 2006; 71(2):331-41. DOI: 10.1016/j.cardiores.2006.01.022. View

Moll F, Walter M, Rezende F, Helfinger V, Vasconez E, De Oliveira T . NoxO1 Controls Proliferation of Colon Epithelial Cells. Front Immunol. 2018; 9:973. PMC: 5951971. DOI: 10.3389/fimmu.2018.00973. View

Mori M, Li G, Hashimoto M, Nishio A, Tomozawa H, Suzuki N . Pivotal Advance: Eosinophilia in the MES rat strain is caused by a loss-of-function mutation in the gene for cytochrome b(-245), alpha polypeptide (Cyba). J Leukoc Biol. 2009; 86(3):473-8. DOI: 10.1189/jlb.1108715. View

Wang X, Elksnis A, Wikstrom P, Walum E, Welsh N, Carlsson P . The novel NADPH oxidase 4 selective inhibitor GLX7013114 counteracts human islet cell death in vitro. PLoS One. 2018; 13(9):e0204271. PMC: 6161897. DOI: 10.1371/journal.pone.0204271. View

10.

Zielonka J, Cheng G, Zielonka M, Ganesh T, Sun A, Joseph J . High-throughput assays for superoxide and hydrogen peroxide: design of a screening workflow to identify inhibitors of NADPH oxidases. J Biol Chem. 2014; 289(23):16176-89. PMC: 4047388. DOI: 10.1074/jbc.M114.548693. View

11.

Vogel J, Kruse C, Zhang M, Schroder K . Nox4 supports proper capillary growth in exercise and retina neo-vascularization. J Physiol. 2015; 593(9):2145-54. PMC: 4422568. DOI: 10.1113/jphysiol.2014.284901. View

12.

Csanyi G, Cifuentes-Pagano E, Al Ghouleh I, Ranayhossaini D, Egana L, Lopes L . Nox2 B-loop peptide, Nox2ds, specifically inhibits the NADPH oxidase Nox2. Free Radic Biol Med. 2011; 51(6):1116-25. PMC: 3204933. DOI: 10.1016/j.freeradbiomed.2011.04.025. View

13.

Nakano Y, Longo-Guess C, Bergstrom D, Nauseef W, Jones S, Banfi B . Mutation of the Cyba gene encoding p22phox causes vestibular and immune defects in mice. J Clin Invest. 2008; 118(3):1176-85. PMC: 2248803. DOI: 10.1172/JCI33835. View

14.

Jaquet V, Marcoux J, Forest E, Leidal K, McCormick S, Westermaier Y . NADPH oxidase (NOX) isoforms are inhibited by celastrol with a dual mode of action. Br J Pharmacol. 2011; 164(2b):507-20. PMC: 3188888. DOI: 10.1111/j.1476-5381.2011.01439.x. View

15.

Kleikers P, Dao V, Gob E, Hooijmans C, Debets J, van Essen H . SFRR-E Young Investigator AwardeeNOXing out stroke: Identification of NOX4 and 5as targets in blood-brain-barrier stabilisation and neuroprotection. Free Radic Biol Med. 2015; 75 Suppl 1:S16. DOI: 10.1016/j.freeradbiomed.2014.10.593. View

16.

Takac I, Schroder K, Zhang L, Lardy B, Anilkumar N, Lambeth J . The E-loop is involved in hydrogen peroxide formation by the NADPH oxidase Nox4. J Biol Chem. 2011; 286(15):13304-13. PMC: 3075677. DOI: 10.1074/jbc.M110.192138. View

17.

Paffenholz R, Bergstrom R, Pasutto F, Wabnitz P, Munroe R, Jagla W . Vestibular defects in head-tilt mice result from mutations in Nox3, encoding an NADPH oxidase. Genes Dev. 2004; 18(5):486-91. PMC: 374230. DOI: 10.1101/gad.1172504. View

18.

Kofler P, Pircher H, von Grafenstein S, Diener T, Holl M, Liedl K . Characterisation of Nox4 inhibitors from edible plants. Planta Med. 2013; 79(3-4):244-52. DOI: 10.1055/s-0032-1328129. View

19.

Rezende F, Moll F, Walter M, Helfinger V, Hahner F, Janetzko P . The NADPH organizers NoxO1 and p47phox are both mediators of diabetes-induced vascular dysfunction in mice. Redox Biol. 2017; 15:12-21. PMC: 5723277. DOI: 10.1016/j.redox.2017.11.014. View

20.

Altenhofer S, Radermacher K, Kleikers P, Wingler K, Schmidt H . Evolution of NADPH Oxidase Inhibitors: Selectivity and Mechanisms for Target Engagement. Antioxid Redox Signal. 2014; 23(5):406-27. PMC: 4543484. DOI: 10.1089/ars.2013.5814. View