Proteomic Analysis Reveals the Damaging Role of Low Redox Laccase from Yersinia Enterocolitica Strain 8081 in the Midgut of Helicoverpa Armigera

Overview

Journal Biotechnol Lett

Specialties Biochemistry
Biotechnology

Date 2020 May 31

PMID 32472187

Citations 6

Authors

Shruti Ahlawat

Deepti Singh

Asha Yadav

Amarjeet Kumar Singh

Jugsharan Singh Virdi

Krishna Kant Sharma

Affiliations

Soon will be listed here.

Abstract

Objective: Earlier, we have found that the enteropathogenic Yersinia enterocolitica have evolved the survival mechanisms that regulate the expression of laccase-encoding genes in the gut. The present study aims to characterize the purified recombinant laccase from Y. enterocolitica strain 8081 biovar 1B and understand its effect on the midgut of cotton bollworm, Helicoverpa armigera (Hübner) larvae.

Results: The recombinant laccase protein showed high purity fold and low molecular mass (~ 43 kDa). H. armigera larvae fed with laccase protein showed a significant decrease in body weight and damage in the midgut. Further, transmission electron microscopy (TEM) studies revealed the negative effect of laccase protein on trachea, malpighian tubules, and villi of the insect. The proteome comparison between control and laccase-fed larvae of cotton bollworm showed significant expression of proteolytic enzymes, oxidoreductases, cytoskeletal proteins, ribosomal proteins; and proteins for citrate (TCA cycle) cycle, glycolysis, stress response, cell redox homeostasis, xenobiotic degradation, and insect defence. Moreover, it also resulted in the reduction of antioxidants, increased melanization (insect innate immune response), and enhanced free radical generation.

Conclusions: All these data collectively suggest that H. armigera (Hübner) larvae can be used to study the effect of microbes and their metabolites on the host physiology, anatomy, and survival.

Citing Articles

Proteome profiling, biochemical and histological analysis of diclofenac-induced liver toxicity in Yersinia enterocolitica and Lactobacillus fermentum fed rat model: a comparative analysis.

Ahlawat S, Mohan H, Sharma K Biotechnol Lett. 2024; 46(5):807-826.

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Lepidopteran insects: emerging model organisms to study infection by enteropathogens.

Ahlawat S, Sharma K Folia Microbiol (Praha). 2022; 68(2):181-196.

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Contribution of insect gut microbiota and their associated enzymes in insect physiology and biodegradation of pesticides.

Jaffar S, Ahmad S, Lu Y Front Microbiol. 2022; 13:979383.

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Probiotics, prebiotics and synbiotics: Safe options for next-generation therapeutics.

Yadav M, Kumari I, Singh B, Sharma K, Tiwari S Appl Microbiol Biotechnol. 2022; 106(2):505-521.

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Infected insect gut reveals differentially expressed proteins for cellular redox, metal resistance and secretion system in Yersinia enterocolitica-Helicoverpa armigera pathogenic model.

Ahlawat S, Singh A, Shankar A, Yadav A, Sharma K Biotechnol Lett. 2021; 43(9):1845-1867.

PMID: 34165641 DOI: 10.1007/s10529-021-03157-3.

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