Phytosterol, Lipid and Phenolic Composition, and Biological Activities of Guava Seed Oil

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2020 May 31

PMID 32471050

Citations 8

Authors

Adchara Prommaban

Niramon Utama-Ang

Anan Chaikitwattana

Chairat Uthaipibull

John B Porter

Somdet Srichairatanakool

Affiliations

Soon will be listed here.

Abstract

Plant seeds have been found to contain bioactive compounds that have potential nutraceutical benefits. Guava seeds () are by-products in the beverage and juice industry; however, they can be utilized for a variety of commercial purposes. This study was designed to analyze the phytochemicals of the -hexane extract of guava seed oil (GSO), to study its free-radical scavenging activity, and to monitor the changes in serum lipids and fatty acid profiles in rats that were fed GSO. The GSO was analyzed for phytochemicals using chromatographic methods. It was also tested for free-radical scavenging activity in hepatoma and neuroblastoma cells, and analyzed in terms of serum lipids and fatty acids. GSO was found to contain phenolic compounds (e.g., chlorogenic acid and its derivatives) and phytosterols (e.g., stimasterol, β-sitosterol and campesterol), and exerted radical-scavenging activity in cell cultures in a concentration-dependent manner. Long-term consumption of GSO did not increase cholesterol and triglyceride levels in rat serum, but it tended to decrease serum fatty acid levels in a concentration-dependent manner. This is the first study to report on the lipid, phytosterol and phenolic compositions, antioxidant activity, and the hepato- and neuro-protection of hydrogen peroxide-induced oxidative stress levels in the GSO extract.

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References

Salib J, Michael H . Cytotoxic phenylethanol glycosides from Psidium guaijava seeds. Phytochemistry. 2004; 65(14):2091-3. DOI: 10.1016/j.phytochem.2004.06.009. View

Simirgiotis M, Benites J, Areche C, Sepulveda B . Antioxidant Capacities and Analysis of Phenolic Compounds in Three Endemic Nolana Species by HPLC-PDA-ESI-MS. Molecules. 2015; 20(6):11490-507. PMC: 6272610. DOI: 10.3390/molecules200611490. View

Morais-Braga M, Carneiro J, Machado A, Dos Santos A, Sales D, Lima L . Psidium guajava L., from ethnobiology to scientific evaluation: Elucidating bioactivity against pathogenic microorganisms. J Ethnopharmacol. 2016; 194:1140-1152. DOI: 10.1016/j.jep.2016.11.017. View

Conde Garcia E, Nascimento V, Santiago Santos A . Inotropic effects of extracts of Psidium guajava L. (guava) leaves on the guinea pig atrium. Braz J Med Biol Res. 2003; 36(5):661-8. DOI: 10.1590/s0100-879x2003000500014. View

Aludatt M, Rababah T, Alhamad M, Al-Mahasneh M, Almajwal A, Gammoh S . A review of phenolic compounds in oil-bearing plants: Distribution, identification and occurrence of phenolic compounds. Food Chem. 2016; 218:99-106. DOI: 10.1016/j.foodchem.2016.09.057. View

Ma J, Zhou X, Fu J, He C, Feng R, Huang M . In Vivo Metabolite Profiling of a Purified Ellagitannin Isolated from Polygonum capitatum in Rats. Molecules. 2016; 21(9). PMC: 6272911. DOI: 10.3390/molecules21091110. View

Yu F, Qian H, Zhang J, Sun J, Ma Z . Simultaneous quantification of eight organic acid components in Artemisia capillaris Thunb (Yinchen) extract using high-performance liquid chromatography coupled with diode array detection and high-resolution mass spectrometry. J Food Drug Anal. 2018; 26(2):788-795. PMC: 9322227. DOI: 10.1016/j.jfda.2017.04.003. View

Garg S, Talwar G, Upadhyay S . Comparison of extraction procedures on the immunocontraceptive activity of neem seed extracts. J Ethnopharmacol. 1994; 44(2):87-92. DOI: 10.1016/0378-8741(94)90073-6. View

Pangjit K, Udomsuk L, Upanan S, Pongjanta A, Chansiw N, Srichairatanakool S . Iron-Chelating and Anti-Hemolytic Properties of Ethanolic Extract of Lotus (Nelumbonucifera Gaertn) Leaves. J Med Assoc Thai. 2016; 99 Suppl 1:S58-66. View

10.

Adesida A, Farombi E . Free radical scavenging activities of guava extract in vitro. Afr J Med Med Sci. 2013; 41 Suppl:81-90. View

11.

Kayano S, Yamada N, Suzuki T, Ikami T, Shioaki K, Kikuzaki H . Quantitative evaluation of antioxidant components in prunes (Prunus domestica L.). J Agric Food Chem. 2003; 51(5):1480-5. DOI: 10.1021/jf025929c. View

12.

Kim S, Park R, Jeon H, Kwon Y, Chun W . Neuroprotective effects of 3,5-dicaffeoylquinic acid on hydrogen peroxide-induced cell death in SH-SY5Y cells. Phytother Res. 2005; 19(3):243-5. DOI: 10.1002/ptr.1652. View

13.

Asadi F, Shahriari A, Chahardah-Cheric M . Effect of long-term optional ingestion of canola oil, grape seed oil, corn oil and yogurt butter on serum, muscle and liver cholesterol status in rats. Food Chem Toxicol. 2010; 48(8-9):2454-7. DOI: 10.1016/j.fct.2010.06.012. View

14.

Pelegrini P, Murad A, Silva L, Dos Santos R, Costa F, Tagliari P . Identification of a novel storage glycine-rich peptide from guava (Psidium guajava) seeds with activity against Gram-negative bacteria. Peptides. 2008; 29(8):1271-9. DOI: 10.1016/j.peptides.2008.03.013. View

15.

Menichini F, Losi L, Bonesi M, Pugliese A, Loizzo M, Tundis R . Chemical profiling and in vitro biological effects of Cardiospermum halicacabum L. (Sapindaceae) aerial parts and seeds for applications in neurodegenerative disorders. J Enzyme Inhib Med Chem. 2013; 29(5):677-85. DOI: 10.3109/14756366.2013.840614. View

16.

Hossain M, Rai D, Brunton N, Martin-Diana A, Barry-Ryan C . Characterization of phenolic composition in Lamiaceae spices by LC-ESI-MS/MS. J Agric Food Chem. 2010; 58(19):10576-81. DOI: 10.1021/jf102042g. View

17.

Lee S, Lee H, Nam T, Eom S, Heo H, Lee C . Neuroprotective effect of caffeoylquinic acids from Artemisia princeps Pampanini against oxidative stress-induced toxicity in PC-12 cells. J Food Sci. 2011; 76(2):C250-6. DOI: 10.1111/j.1750-3841.2010.02010.x. View

18.

Gu C, Howell K, Dunshea F, Suleria H . LC-ESI-QTOF/MS Characterisation of Phenolic Acids and Flavonoids in Polyphenol-Rich Fruits and Vegetables and Their Potential Antioxidant Activities. Antioxidants (Basel). 2019; 8(9). PMC: 6770053. DOI: 10.3390/antiox8090405. View

19.

Zahir A, Rahuman A, Kamaraj C, Bagavan A, Elango G, Sangaran A . Laboratory determination of efficacy of indigenous plant extracts for parasites control. Parasitol Res. 2009; 105(2):453-61. DOI: 10.1007/s00436-009-1405-1. View

20.

Arranz S, Cert R, Perez-Jimenez J, Cert A, Saura-Calixto F . Comparison between free radical scavenging capacity and oxidative stability of nut oils. Food Chem. 2015; 110(4):985-90. DOI: 10.1016/j.foodchem.2008.03.021. View