Effect of the 13Tul Antigen from on Splenocytes from Mice

Overview

Journal Parasitology

Specialty Parasitology

Date 2020 May 30

PMID 32466805

Authors

Laura Monica Tasso

Andrea Cecilia Bruballa

Patricia Andrea Garavaglia

Monica Ines Esteva

Maria Cecilia Albareda

Gabriela Andrea Garcia

Affiliations

Soon will be listed here.

Abstract

Trypanosoma cruzi, the etiological agent of Chagas disease, releases factors, including antigens from the trans-sialidase (TS) superfamily, which modulate the host immune responses. Tc13 antigens belong to group IV of TSs and are characterized by C-terminal EPKSA repeats. Here, we studied the effect of the Tc13 antigen from the Tulahuén strain, Tc13Tul, on primary cultures of splenocytes from naïve BALB/c mice. Recombinant Tc13Tul increased the percentage of viable cells and induced B (CD19+) lymphocyte proliferation. Tc13Tul stimulation also induced secretion of non-specific IgM and interferon-γ (IFN-γ). The same effects were induced by Tc13Tul on splenocytes from naïve C3H/HeJ mice. In vivo administration of Tc13Tul to naïve BALB/c mice increased non-specific IgG in sera. In addition, in vitro cultured splenocytes from Tc13Tul-inoculated mice secreted a higher basal level of non-specific IgM than controls and the in vitro Tc13Tul stimulation of these cells showed an enhanced effect on IgM and IFN-γ secretion. Our results indicate that Tc13Tul may participate in the early immunity in T. cruzi infection by favouring immune system evasion through B-cell activation and non-specific Ig secretion. In contrast, as IFN-γ is an important factor involved in T. cruzi resistance, this may be considered a Tc13Tul effect in favour of the host.

References

Martins G, Vieira L, Cunha F, Silva J . Gamma interferon modulates CD95 (Fas) and CD95 ligand (Fas-L) expression and nitric oxide-induced apoptosis during the acute phase of Trypanosoma cruzi infection: a possible role in immune response control. Infect Immun. 1999; 67(8):3864-71. PMC: 96666. DOI: 10.1128/IAI.67.8.3864-3871.1999. View

Garcia G, Joensen L, Bua J, Ainciart N, Perry S, Ruiz A . Trypanosoma cruzi: molecular identification and characterization of new members of the Tc13 family. Description of the interaction between the Tc13 antigen from Tulahuén strain and the second extracellular loop of the beta(1)-adrenergic receptor. Exp Parasitol. 2003; 103(3-4):112-9. DOI: 10.1016/s0014-4894(03)00087-0. View

Garcia G, Arnaiz M, Laucella S, Esteva M, Ainciart N, Riarte A . Immunological and pathological responses in BALB/c mice induced by genetic administration of Tc 13 Tul antigen of Trypanosoma cruzi. Parasitology. 2006; 132(Pt 6):855-66. DOI: 10.1017/S0031182005009753. View

Aridgides D, Salvador R, PereiraPerrin M . Trypanosoma cruzi coaxes cardiac fibroblasts into preventing cardiomyocyte death by activating nerve growth factor receptor TrkA. PLoS One. 2013; 8(2):e57450. PMC: 3578799. DOI: 10.1371/journal.pone.0057450. View

Leguizamon M, Mocetti E, Garcia Rivello H, Argibay P, Campetella O . Trans-sialidase from Trypanosoma cruzi induces apoptosis in cells from the immune system in vivo. J Infect Dis. 1999; 180(4):1398-402. DOI: 10.1086/315001. View

Garcia G, Arnaiz M, Esteva M, Laucella S, Garavaglia P, Ibarra S . Evaluation of immune responses raised against Tc13 antigens of Trypanosoma cruzi in the outcome of murine experimental infection. Parasitology. 2007; 135(3):347-57. DOI: 10.1017/S0031182007003873. View

Bryan M, Guyach S, Norris K . Specific humoral immunity versus polyclonal B cell activation in Trypanosoma cruzi infection of susceptible and resistant mice. PLoS Negl Trop Dis. 2010; 4(7):e733. PMC: 2897841. DOI: 10.1371/journal.pntd.0000733. View

Peralta J, Teixeira M, Shreffler W, Pereira J, Burns Jr J, Sleath P . Serodiagnosis of Chagas' disease by enzyme-linked immunosorbent assay using two synthetic peptides as antigens. J Clin Microbiol. 1994; 32(4):971-4. PMC: 267164. DOI: 10.1128/jcm.32.4.971-974.1994. View

Bermejo D, Amezcua Vesely M, Khan M, Acosta Rodriguez E, Montes C, Merino M . Trypanosoma cruzi infection induces a massive extrafollicular and follicular splenic B-cell response which is a high source of non-parasite-specific antibodies. Immunology. 2010; 132(1):123-33. PMC: 3015082. DOI: 10.1111/j.1365-2567.2010.03347.x. View

10.

Buscaglia C, Campetella O, Leguizamon M, Frasch A . The repetitive domain of Trypanosoma cruzi trans-sialidase enhances the immune response against the catalytic domain. J Infect Dis. 1998; 177(2):431-6. DOI: 10.1086/514199. View

11.

Buschiazzo A, Amaya M, Cremona M, Frasch A, Alzari P . The crystal structure and mode of action of trans-sialidase, a key enzyme in Trypanosoma cruzi pathogenesis. Mol Cell. 2002; 10(4):757-68. DOI: 10.1016/s1097-2765(02)00680-9. View

12.

Reina-San-Martin B, Degrave W, Rougeot C, Cosson A, Chamond N, Cordeiro-da-Silva A . A B-cell mitogen from a pathogenic trypanosome is a eukaryotic proline racemase. Nat Med. 2000; 6(8):890-7. DOI: 10.1038/78651. View

13.

Salvador R, Aridgides D, PereiraPerrin M . Parasite-derived neurotrophic factor/trans-sialidase of Trypanosoma cruzi links neurotrophic signaling to cardiac innate immune response. Infect Immun. 2014; 82(9):3687-96. PMC: 4187833. DOI: 10.1128/IAI.02098-14. View

14.

Mucci J, Risso M, Leguizamon M, Frasch A, Campetella O . The trans-sialidase from Trypanosoma cruzi triggers apoptosis by target cell sialylation. Cell Microbiol. 2006; 8(7):1086-95. DOI: 10.1111/j.1462-5822.2006.00689.x. View

15.

Todeschini A, Nunes M, Pires R, Lopes M, Previato J, Mendonca-Previato L . Costimulation of host T lymphocytes by a trypanosomal trans-sialidase: involvement of CD43 signaling. J Immunol. 2002; 168(10):5192-8. DOI: 10.4049/jimmunol.168.10.5192. View

16.

Romero-Ramirez S, Navarro-Hernandez I, Cervantes-Diaz R, Sosa-Hernandez V, Acevedo-Ochoa E, Kleinberg-Bild A . Innate-like B cell subsets during immune responses: Beyond antibody production. J Leukoc Biol. 2018; 105(5):843-856. DOI: 10.1002/JLB.MR0618-227R. View

17.

Gao W, Wortis H, Pereira M . The Trypanosoma cruzi trans-sialidase is a T cell-independent B cell mitogen and an inducer of non-specific Ig secretion. Int Immunol. 2002; 14(3):299-308. DOI: 10.1093/intimm/14.3.299. View

18.

Bao Y, Liu X, Han C, Xu S, Xie B, Zhang Q . Identification of IFN-γ-producing innate B cells. Cell Res. 2013; 24(2):161-76. PMC: 3915900. DOI: 10.1038/cr.2013.155. View

19.

Da Silva A, Espinoza A, Taibi A, Ouaissi A, Minoprio P . A 24,000 MW Trypanosoma cruzi antigen is a B-cell activator. Immunology. 1998; 94(2):189-96. PMC: 1364204. DOI: 10.1046/j.1365-2567.1998.00498.x. View

20.

Bontempi E, Garcia G, Buschiazzo A, Henriksson J, Pravia C, Ruiz A . The tyrosine aminotransferase from Trypanosoma rangeli: sequence and genomic characterization. FEMS Microbiol Lett. 2000; 189(2):253-7. DOI: 10.1111/j.1574-6968.2000.tb09239.x. View