Elastin-derived Peptide VGVAPG Decreases Differentiation of Mouse Embryo Fibroblast (3T3-L1) Cells into Adipocytes

Overview

Journal Adipocyte

Specialties Cell Biology
Endocrinology
Physiology

Date 2020 May 29

PMID 32463311

Citations 5

Authors

Konrad A Szychowski

Bartosz Skora

Jakub Tobiasz

Jan Gminski

Affiliations

Soon will be listed here.

Abstract

Abbreviations: DMSO: dimethyl sulphoxide; EBP: elastin-binding protein; EDPs: elastin-derived peptides; FBS: foetal bovine serum; : gene for beta-galactosidase; LDL: low-density-lipoprotein; PAI-1 (Serpin E1): plasminogen activator inhibitor-1; PBS: phosphate-buffered saline; PPARγ: peroxisome proliferator-activated receptor gamma; Pref-1: preadipocyte factor 1; ROS: reactive oxygen species; VEGF-A: vascular endothelial growth factor-A; VGVAPG: Val-Gly-Val-Ala-Pro-Gly; β-Gal: beta-galactosidase; ORO: oil red O; IBMX: 3-isobutyl-1-methylxanthine; HDCFDA: 2',7'-dichlorodihydrofluorescein diacetate; DMEM: Dulbecco's Modified Eagle's Medium; VVGPGA: Val-Val-Gly-Pro-Gly-Ala.

Citing Articles

Interaction Between Aging-Related Elastin-Derived Peptide (VGVAPG) and Sirtuin 2 and its Impact on Functions of Human Neuron Cells in an In Vitro Model.

Skora B, Piechowiak T, Szychowski K Mol Neurobiol. 2024; 62(1):819-831.

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Sialic acids cleavage induced by elastin-derived peptides impairs the interaction between insulin and its receptor in adipocytes 3T3-L1.

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Elastin-Derived VGVAPG Fragment Decorated Cell-Penetrating Peptide with Improved Gene Delivery Efficacy.

Shen W, Tian D, Fu L, Jin B, Liu Y, Xu Y Pharmaceutics. 2023; 15(2).

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Immune cell infiltration and the genes associated with ligamentum flavum hypertrophy: Identification and validation.

Duan Y, Ni S, Zhao K, Qian J, Hu X Front Cell Dev Biol. 2022; 10:914781.

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Elastin-Derived Peptides in the Central Nervous System: Friend or Foe.

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References

Fasshauer M, Paschke R, Stumvoll M . Adiponectin, obesity, and cardiovascular disease. Biochimie. 2004; 86(11):779-84. DOI: 10.1016/j.biochi.2004.09.016. View

Ochieng J, Furtak V, Lukyanov P . Extracellular functions of galectin-3. Glycoconj J. 2004; 19(7-9):527-35. DOI: 10.1023/B:GLYC.0000014082.99675.2f. View

Membrez M, Ammon-Zufferey C, Philippe D, Aprikian O, Monnard I, Mace K . Interleukin-18 protein level is upregulated in adipose tissue of obese mice. Obesity (Silver Spring). 2008; 17(2):393-5. DOI: 10.1038/oby.2008.535. View

Bresalier R, Byrd J, Wang L, Raz A . Colon cancer mucin: a new ligand for the beta-galactoside-binding protein galectin-3. Cancer Res. 1996; 56(19):4354-7. View

Stofkova A . Leptin and adiponectin: from energy and metabolic dysbalance to inflammation and autoimmunity. Endocr Regul. 2009; 43(4):157-68. View

Coelho M, Oliveira T, Fernandes R . Biochemistry of adipose tissue: an endocrine organ. Arch Med Sci. 2013; 9(2):191-200. PMC: 3648822. DOI: 10.5114/aoms.2013.33181. View

Takamura T, Nohara E, Nagai Y, Kobayashi K . Stage-specific effects of a thiazolidinedione on proliferation, differentiation and PPARgamma mRNA expression in 3T3-L1 adipocytes. Eur J Pharmacol. 2001; 422(1-3):23-9. DOI: 10.1016/s0014-2999(01)01053-6. View

Gomes A, Fernandes E, Lima J . Fluorescence probes used for detection of reactive oxygen species. J Biochem Biophys Methods. 2005; 65(2-3):45-80. DOI: 10.1016/j.jbbm.2005.10.003. View

Holmes D, Zachary I . The vascular endothelial growth factor (VEGF) family: angiogenic factors in health and disease. Genome Biol. 2005; 6(2):209. PMC: 551528. DOI: 10.1186/gb-2005-6-2-209. View

10.

Lapis K, Timar J . Role of elastin-matrix interactions in tumor progression. Semin Cancer Biol. 2002; 12(3):209-17. DOI: 10.1016/S1044-579X(02)00024-X. View

11.

Szychowski K, Rombel-Bryzek A, Dolhanczuk-Srodka A, Gminski J . Antiproliferative Effect of Elastin-Derived Peptide VGVAPG on SH-SY5Y Neuroblastoma Cells. Neurotox Res. 2019; 36(3):503-514. PMC: 6745029. DOI: 10.1007/s12640-019-00040-y. View

12.

Green H . Adipose conversion of 3T3 cells depends on a serum factor. Proc Natl Acad Sci U S A. 1978; 75(12):6107-9. PMC: 393127. DOI: 10.1073/pnas.75.12.6107. View

13.

Le Page A, Khalil A, Vermette P, Frost E, Larbi A, Witkowski J . The role of elastin-derived peptides in human physiology and diseases. Matrix Biol. 2019; 84:81-96. DOI: 10.1016/j.matbio.2019.07.004. View

14.

Gong H, Ni Y, Guo X, Fei L, Pan X, Guo M . Resistin promotes 3T3-L1 preadipocyte differentiation. Eur J Endocrinol. 2004; 150(6):885-92. DOI: 10.1530/eje.0.1500885. View

15.

Khatiwala C, Kim P, Peyton S, Putnam A . ECM compliance regulates osteogenesis by influencing MAPK signaling downstream of RhoA and ROCK. J Bone Miner Res. 2008; 24(5):886-98. PMC: 2672206. DOI: 10.1359/jbmr.081240. View

16.

Haugen F, Jorgensen A, Drevon C, Trayhurn P . Inhibition by insulin of resistin gene expression in 3T3-L1 adipocytes. FEBS Lett. 2001; 507(1):105-8. DOI: 10.1016/s0014-5793(01)02968-4. View

17.

Blood C, Sasse J, Brodt P, Zetter B . Identification of a tumor cell receptor for VGVAPG, an elastin-derived chemotactic peptide. J Cell Biol. 1988; 107(5):1987-93. PMC: 2115331. DOI: 10.1083/jcb.107.5.1987. View

18.

Brophy C, Reilly J, Smith G, Tilson M . The role of inflammation in nonspecific abdominal aortic aneurysm disease. Ann Vasc Surg. 1991; 5(3):229-33. DOI: 10.1007/BF02329378. View

19.

Wang Y, Nangia-Makker P, Tait L, Balan V, Hogan V, Pienta K . Regulation of prostate cancer progression by galectin-3. Am J Pathol. 2009; 174(4):1515-23. PMC: 2671381. DOI: 10.2353/ajpath.2009.080816. View

20.

Ezure T, Amano S . Increment of subcutaneous adipose tissue is associated with decrease of elastic fibres in the dermal layer. Exp Dermatol. 2015; 24(12):924-9. DOI: 10.1111/exd.12816. View