Full-length Transcriptome and Targeted Metabolome Analyses Provide Insights into Defense Mechanisms of Against

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2020 May 29

PMID 32461824

Citations 5

Authors

Chuang Mei

Jie Yang

Peng Yan

Ning Li

Kai Ma

Aisajan Mamat

Liqun Han

Qinglong Dong

Ke Mao

Fengwang Ma

Jixun Wang

Affiliations

Soon will be listed here.

Abstract

is the wild progenitor for many cultivars of domesticated apple and an important germplasm resource for breeding. However, this valuable species faces a significant threat in the areas north of the Tianshan Mountains in China, by the invasion of , a destructive pest of apple trees belonging to the family Buprestidae. Our preliminary study has has shown that there may be resistance to this insect in plants in the field, but the corresponding molecular mechanisms remain unclear. In this study, we compared the response of insect-resistant and insect-susceptible plants of to insect feeding using full-length transcriptome and targeted metabolome. 112,103 non-chimeric full-length reads (FLNC) totaling 10.52 Gb of data were generating with Pacific Biosciences SingleMolecule, Real-Time (PacBio SMRT) sequencing. A total of 130.06 Gb data of long reads were acquired with an Illumina HiSeq. Function annotation indicated that the different expressed genes (DEGs) were mainly involved in signal transduction pathway of plant hormones and in the synthesis of compounds such as terpenes, quinones, flavonoids, and jasmonic acid. Through targeted metabolome analysis resistant strains showed higher levels of trans-cinnamic acid, caffeine and ferulic acid after pest infestation. This study helps to decipher the transcriptional changes and related signaling paths in after an insect feeding, which lays a foundation for further research on molecular mechanisms of insect resistance in apples.

Citing Articles

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References

Ren P, Meng Y, Li B, Ma X, Si E, Lai Y . Molecular Mechanisms of Acclimatization to Phosphorus Starvation and Recovery Underlying Full-Length Transcriptome Profiling in Barley ( L.). Front Plant Sci. 2018; 9:500. PMC: 5915550. DOI: 10.3389/fpls.2018.00500. View

Thireault C, Shyu C, Yoshida Y, Aubin B, Campos M, Howe G . Repression of jasmonate signaling by a non-TIFY JAZ protein in Arabidopsis. Plant J. 2015; 82(4):669-79. DOI: 10.1111/tpj.12841. View

Xia W, Yu H, Cao P, Luo J, Wang N . Identification of TIFY Family Genes and Analysis of Their Expression Profiles in Response to Phytohormone Treatments and Infection in Poplar. Front Plant Sci. 2017; 8:493. PMC: 5380741. DOI: 10.3389/fpls.2017.00493. View

Hackl T, Hedrich R, Schultz J, Forster F . proovread: large-scale high-accuracy PacBio correction through iterative short read consensus. Bioinformatics. 2014; 30(21):3004-11. PMC: 4609002. DOI: 10.1093/bioinformatics/btu392. View

Deng Y, Zheng H, Yan Z, Liao D, Li C, Zhou J . Full-Length Transcriptome Survey and Expression Analysis of to Discover Putative Genes Related to Aurantio-Obtusin Biosynthesis, Seed Formation and Development, and Stress Response. Int J Mol Sci. 2018; 19(9). PMC: 6163539. DOI: 10.3390/ijms19092476. View

Barah P, Bones A . Multidimensional approaches for studying plant defence against insects: from ecology to omics and synthetic biology. J Exp Bot. 2014; 66(2):479-93. DOI: 10.1093/jxb/eru489. View

Ma X, Cai Z, Liu W, Ge S, Tang L . Identification, genealogical structure and population genetics of S-alleles in Malus sieversii, the wild ancestor of domesticated apple. Heredity (Edinb). 2017; 119(3):185-196. PMC: 5564378. DOI: 10.1038/hdy.2017.28. View

Liu Q, Wang X, Tzin V, Romeis J, Peng Y, Li Y . Combined transcriptome and metabolome analyses to understand the dynamic responses of rice plants to attack by the rice stem borer Chilo suppressalis (Lepidoptera: Crambidae). BMC Plant Biol. 2016; 16(1):259. PMC: 5142284. DOI: 10.1186/s12870-016-0946-6. View

Han Y, Wang Y, Bi J, Yang X, Huang Y, Zhao X . Constitutive and induced activities of defense-related enzymes in aphid-resistant and aphid-susceptible cultivars of wheat. J Chem Ecol. 2009; 35(2):176-82. DOI: 10.1007/s10886-009-9589-5. View

10.

Zhu C, Li X, Zheng J . Transcriptome profiling using Illumina- and SMRT-based RNA-seq of hot pepper for in-depth understanding of genes involved in CMV infection. Gene. 2018; 666:123-133. DOI: 10.1016/j.gene.2018.05.004. View

11.

Mao Y, Cai W, Wang J, Hong G, Tao X, Wang L . Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol. Nat Biotechnol. 2007; 25(11):1307-13. DOI: 10.1038/nbt1352. View

12.

Howe G, Jander G . Plant immunity to insect herbivores. Annu Rev Plant Biol. 2007; 59:41-66. DOI: 10.1146/annurev.arplant.59.032607.092825. View

13.

Ebel C, BenFeki A, Hanin M, Solano R, Chini A . Characterization of wheat (Triticum aestivum) TIFY family and role of Triticum Durum TdTIFY11a in salt stress tolerance. PLoS One. 2018; 13(7):e0200566. PMC: 6051620. DOI: 10.1371/journal.pone.0200566. View

14.

Chao Y, Yuan J, Li S, Jia S, Han L, Xu L . Analysis of transcripts and splice isoforms in red clover (Trifolium pratense L.) by single-molecule long-read sequencing. BMC Plant Biol. 2018; 18(1):300. PMC: 6258457. DOI: 10.1186/s12870-018-1534-8. View

15.

Pan X, Welti R, Wang X . Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography-mass spectrometry. Nat Protoc. 2010; 5(6):986-92. DOI: 10.1038/nprot.2010.37. View

16.

Chaman M, Copaja S, Argandona V . Relationships between salicylic acid content, phenylalanine ammonia-lyase (PAL) activity, and resistance of barley to aphid infestation. J Agric Food Chem. 2003; 51(8):2227-31. DOI: 10.1021/jf020953b. View

17.

Torres M, Dangl J, Jones J . Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response. Proc Natl Acad Sci U S A. 2002; 99(1):517-22. PMC: 117592. DOI: 10.1073/pnas.012452499. View

18.

Marino D, Dunand C, Puppo A, Pauly N . A burst of plant NADPH oxidases. Trends Plant Sci. 2011; 17(1):9-15. DOI: 10.1016/j.tplants.2011.10.001. View

19.

Francescato L, Debenedetti S, Schwanz T, Bassani V, Henriques A . Identification of phenolic compounds in Equisetum giganteum by LC-ESI-MS/MS and a new approach to total flavonoid quantification. Talanta. 2013; 105:192-203. DOI: 10.1016/j.talanta.2012.11.072. View

20.

Wojakowska A, Piasecka A, Garcia-Lopez P, Zamora-Natera F, Krajewski P, Marczak L . Structural analysis and profiling of phenolic secondary metabolites of Mexican lupine species using LC-MS techniques. Phytochemistry. 2013; 92:71-86. DOI: 10.1016/j.phytochem.2013.04.006. View