Comprehensive Analysis of T-cell Receptor Repertoire in Patients with Acute Coronary Syndrome by High-throughput Sequencing

Overview

Journal BMC Cardiovasc Disord

Publisher Biomed Central

Specialty Cardiology & Vascular Diseases

Date 2020 May 29

PMID 32460698

Citations 1

Authors

Sudong Liu

Zhixiong Zhong

Wei Zhong

Ruiqiang Weng

Jing Liu

Xiaodong Gu

Yongyu Chen

Affiliations

Soon will be listed here.

Abstract

Background: This study aims to investigate the T-cell receptor (TCR) repertoire in patients with acute coronary syndrome (ACS).

Methods: The TCR repertoires of 9 unstable angina patients (UA), 14 acute myocardial infarction patients (AMI) and 9 normal coronary artery (NCA) patients were profiled using high-throughput sequencing (HTS). The clonal diversity of the TCR repertoires in different groups was analyzed, as well as the frequencies of variable (V), diversity (D) and joining(J) gene segments.

Results: ACS patients including UA and AMI, showed reduced TCRβ diversity than NCA patients. ACS patients presented higher levels of clonal expansion. The clonotype overlap of complementarity determining region 3(CDR3) was significantly varied between different groups. A total of 10 V genes and 1 J gene were differently utilized between ACS and NCA patients. We identified some shared CDR3 amino acid sequences that were presented in ACS but not in NCA patients.

Conclusions: This study revealed the distinct TCR repertoires in patients with ACS and demonstrated the presence of disease associated T-cell clonotypes. These findings suggested a role of T cells in ACS and provided a new way to explore the mechanisms of ACS.

Citing Articles

T Cells in Atherosclerosis: Key Players in the Pathogenesis of Vascular Disease.

Hinkley H, Counts D, VonCanon E, Lacy M Cells. 2023; 12(17).

PMID: 37681883 PMC: 10486666. DOI: 10.3390/cells12172152.

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