Carnosic Acid Exhibits Antiproliferative and Proapoptotic Effects in Tumoral NCI-H460 and Nontumoral IMR-90 Lung Cells
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Toxicology
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Carnosic acid (CA) is a phenolic diterpene with many important biological activities including antimicrobial, antioxidant, anti-inflammatory properties, and anti-proliferative properties. The aim of the present study was to investigate cytotoxic activity, cell cycle, apoptotic, and molecular effects attributed to CA in non-tumoral IMR-90 (human fetal lung fibroblasts), as well as tumoral NCI-H460 (human non-small-cell lung cancer) cell lines. Cell proliferation was evaluated by Real-Time Cell Analysis system, while apoptosis and cell cycle were assessed using flow cytometry. RT-qPCR was used to estimate the relative expression of genes involved in cell cycle regulation, DNA damage and repair, and apoptosis induction. CA inhibited proliferation of IMR-90 and NCI-H460 cells via cell cycle arrest at G/G and G/M phases, according to the treatment concentration. The mRNA levels of genes encoding cyclins A2, B1, and B2 were downregulated in response to CA treatment of IMR-90 cells. Apoptosis was induced and proapoptotic gene was upregulated in both cell lines. mRNA levels of genes , and were not markedly changed following CA treatments. Although CA exerted antiproliferative activity against NCI-H460 tumor cells, this phytochemical induced toxic effects in non-tumoral cells, and thus needs to be considered carefully prior to pharmacological use therapeutically.
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Sirajudeen F, Bou Malhab L, Bustanji Y, Shahwan M, Alzoubi K, Semreen M Biomol Ther (Seoul). 2023; 32(1):38-55.
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Singh N, Singh Yadav S Curr Res Food Sci. 2022; 5:1845-1872.
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Sufianova G, Gareev I, Beylerli O, Wu J, Shumadalova A, Sufianov A Front Cell Dev Biol. 2022; 10:1011435.
PMID: 36172282 PMC: 9512088. DOI: 10.3389/fcell.2022.1011435.
Acquaviva R, Malfa G, Loizzo M, Xiao J, Bianchi S, Tundis R Molecules. 2022; 27(15).
PMID: 35897965 PMC: 9330018. DOI: 10.3390/molecules27154791.