A Highly Sensitive Amperometric Glutamate Oxidase Microbiosensor Based on a Reduced Graphene Oxide/Prussian Blue Nanocube/Gold Nanoparticle Composite Film-Modified Pt Electrode

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2020 May 28

PMID 32455706

Citations 7

Authors

Jing Chen

Qiwen Yu

Wei Fu

Xing Chen

Quan Zhang

Shurong Dong

Hang Chen

Shaomin Zhang

Affiliations

Soon will be listed here.

Abstract

A simple method that relies only on an electrochemical workstation has been investigated to fabricate a highly sensitive glutamate microbiosensor for potential neuroscience applications. In this study, in order to develop the highly sensitive glutamate electrode, a 100 µm platinum wire was modified by the electrochemical deposition of gold nanoparticles, Prussian blue nanocubes, and reduced graphene oxide sheets, which increased the electroactive surface area; and the chitosan layer, which provided a suitable environment to bond the glutamate oxidase. The optimization of the fabrication procedure and analytical conditions is described. The modified electrode was characterized using field emission scanning electron microscopy, impedance spectroscopy, and cyclic voltammetry. The results exhibited its excellent sensitivity for glutamate detection (LOD = 41.33 nM), adequate linearity (50 nM-40 µM), ascendant reproducibility (RSD = 4.44%), and prolonged stability (more than 30 repetitive potential sweeps, two-week lifespan). Because of the important role of glutamate in neurotransmission and brain function, this small-dimension, high-sensitivity glutamate electrode is a promising tool in neuroscience research.

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References

Paul I, Skolnick P . Glutamate and depression: clinical and preclinical studies. Ann N Y Acad Sci. 2003; 1003:250-72. DOI: 10.1196/annals.1300.016. View

Cao L, Liu Y, Zhang B, Lu L . In situ controllable growth of Prussian blue nanocubes on reduced graphene oxide: facile synthesis and their application as enhanced nanoelectrocatalyst for H2O2 reduction. ACS Appl Mater Interfaces. 2010; 2(8):2339-46. DOI: 10.1021/am100372m. View

Swanson C, Bures M, Johnson M, Linden A, Monn J, Schoepp D . Metabotropic glutamate receptors as novel targets for anxiety and stress disorders. Nat Rev Drug Discov. 2005; 4(2):131-44. DOI: 10.1038/nrd1630. View

Jagadale A, Zhou X, Blaisdell D, Yang S . Carbon nanofibers (CNFs) supported cobalt- nickel sulfide (CoNiS) nanoparticles hybrid anode for high performance lithium ion capacitor. Sci Rep. 2018; 8(1):1602. PMC: 5785478. DOI: 10.1038/s41598-018-19787-z. View

Ricci F, Palleschi G . Sensor and biosensor preparation, optimisation and applications of Prussian Blue modified electrodes. Biosens Bioelectron. 2005; 21(3):389-407. DOI: 10.1016/j.bios.2004.12.001. View

Galvan A, Smith Y, Wichmann T . Continuous monitoring of intracerebral glutamate levels in awake monkeys using microdialysis and enzyme fluorometric detection. J Neurosci Methods. 2003; 126(2):175-85. DOI: 10.1016/s0165-0270(03)00092-x. View

Javitt D . Glutamate as a therapeutic target in psychiatric disorders. Mol Psychiatry. 2004; 9(11):984-97, 979. DOI: 10.1038/sj.mp.4001551. View

Carlsson A, Waters N, Tedroff J, Nilsson M, Carlsson M . Interactions between monoamines, glutamate, and GABA in schizophrenia: new evidence. Annu Rev Pharmacol Toxicol. 2001; 41:237-60. DOI: 10.1146/annurev.pharmtox.41.1.237. View

Hamdan S, Mohd Zain A . In vivo Electrochemical Biosensor for Brain Glutamate Detection: A Mini Review. Malays J Med Sci. 2015; 21(Spec Issue):12-26. PMC: 4405803. View

10.

Tian F, Gourine A, Huckstepp R, Dale N . A microelectrode biosensor for real time monitoring of L-glutamate release. Anal Chim Acta. 2009; 645(1-2):86-91. DOI: 10.1016/j.aca.2009.04.048. View

11.

Dalkiran B, Erden P, Kilic E . Graphene and tricobalt tetraoxide nanoparticles based biosensor for electrochemical glutamate sensing. Artif Cells Nanomed Biotechnol. 2016; 45(2):340-348. DOI: 10.3109/21691401.2016.1153482. View

12.

Basu A, Chattopadhyay P, Roychudhuri U, Chakraborty R . A biosensor based on co-immobilized L-glutamate oxidase and L-glutamate dehydrogenase for analysis of monosodium glutamate in food. Biosens Bioelectron. 2005; 21(10):1968-72. DOI: 10.1016/j.bios.2005.09.011. View

13.

DOrazio P . Biosensors in clinical chemistry. Clin Chim Acta. 2003; 334(1-2):41-69. DOI: 10.1016/s0009-8981(03)00241-9. View

14.

Ozel R, Ispas C, Ganesana M, Leiter J, Andreescu S . Glutamate oxidase biosensor based on mixed ceria and titania nanoparticles for the detection of glutamate in hypoxic environments. Biosens Bioelectron. 2013; 52:397-402. PMC: 3831270. DOI: 10.1016/j.bios.2013.08.054. View

15.

Hu J, Wisetsuwannaphum S, Foord J . Glutamate biosensors based on diamond and graphene platforms. Faraday Discuss. 2014; 172:457-72. DOI: 10.1039/c4fd00032c. View

16.

Batra B, Pundir C . An amperometric glutamate biosensor based on immobilization of glutamate oxidase onto carboxylated multiwalled carbon nanotubes/gold nanoparticles/chitosan composite film modified Au electrode. Biosens Bioelectron. 2013; 47:496-501. DOI: 10.1016/j.bios.2013.03.063. View

17.

Jamal M, Xu J, Razeeb K . Disposable biosensor based on immobilisation of glutamate oxidase on Pt nanoparticles modified Au nanowire array electrode. Biosens Bioelectron. 2010; 26(4):1420-4. DOI: 10.1016/j.bios.2010.07.071. View

18.

Sun D, Sombati S, DeLorenzo R . Glutamate injury-induced epileptogenesis in hippocampal neurons: an in vitro model of stroke-induced "epilepsy". Stroke. 2001; 32(10):2344-50. DOI: 10.1161/hs1001.097242. View

19.

Shah A, de la Flor R, Atkins A, Slone-Murphy J, Dawson L . Development and application of a liquid chromatography/tandem mass spectrometric assay for measurement of N-acetylaspartate, N-acetylaspartylglutamate and glutamate in brain slice superfusates and tissue extracts. J Chromatogr B Analyt Technol Biomed Life Sci. 2008; 876(2):153-8. DOI: 10.1016/j.jchromb.2008.10.012. View

20.

Qin S, Van der Zeyden M, Oldenziel W, Cremers T, Westerink B . Microsensors for in vivo Measurement of Glutamate in Brain Tissue. Sensors (Basel). 2016; 8(11):6860-6884. PMC: 3787420. DOI: 10.3390/s8116860. View