Antipruritic Effect of Ethyl Acetate Extract from in Mice with 2,4-Dinitrofluorobenzene-Induced Atopic Dermatitis

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2020 May 27

PMID 32454868

Citations 6

Authors

Xi Chen

Chan Zhu

Yingge Zhang

Niuniu Yang

Hao Shi

Weiwei Yang

Yan Yang

Jianqiang Liang

Liuzhi Chen

Xueying Zeng

Rijin Cai

Guanyi Wu

Zongxiang Tang

Affiliations

Soon will be listed here.

Abstract

Atopic dermatitis (AD) is a common inflammatory skin disease characterized by intense pruritus and skin lesions. The exact cause of AD is not yet known and the available therapeutic strategies for AD are limited. is commonly used in traditional Chinese medicine as an herb for treating chronic itch. However, the mechanism underlying the antipruritic effects of is not well understood. In the present study, we investigated the antipruritic effect of locally administered ethyl acetate extract from (EAEFC) to 2,4-dinitrofluorobenzene- (DNFB-) induced AD in a mouse model. The scratching behavior, skin thickness, dermatitis score, weight, blood immunoglobulin E (IgE) level, and itch-related cytokine levels were subsequently monitored and evaluated. Results showed that EAEFC treatment attenuated the DNFB-induced AD-like symptoms by alleviating the skin lesions and decreasing the dermatitis score. Hematoxylin and eosin (H&E) and toluidine blue (TB) staining analyses demonstrated that EAEFC mitigated the DNFB-induced increase in skin thickness and prevented the infiltration of mast cells. Behavioral tests showed that EAEFC decreased the DNFB-induced acute and chronic scratching behaviors. Furthermore, EAEFC reduced the levels of itch-related cytokines, such as thymic stromal lymphopoietin (TSLP), interleukin- (IL-) 17, IL-33, and IL-31, and the DNFB-induced boost in serum IgE. Collectively, these results suggest that EAEFC is a potential therapeutic candidate for the treatment of chronic itch in AD.

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