A Simple Isocratic High-perfomance Liquid Chromatography Method for the Simultaneous Determination of Shikonin Derivatives in Some Species Growing Wild in Turkey

Overview

Journal Turk J Pharm Sci

Specialty Pharmacology

Date 2020 May 27

PMID 32454638

Citations 3

Authors

Nuraniye Eruygur

Affiliations

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Abstract

Objectives: Dried roots of species are used in Turkey for the treatment of wounds, inflammation, and depression. In this study, an reversed-phase-liquid chromatographic method with isocratic elution was developed to determine shikonin derivatives in species.

Materials And Methods: The chromatographic separation and quantification was performed on a C18 column (ACE, 150 mm × 4.6 mm, 5 μm), with a mobile phase of acetonitrile and 0.1 M acetic acid (70: 30, v/v) at a flow rate of 1 mL/min, and ultraviolet detection at 520 nm.

Results: Linear behavior was observed over the investigated concentration range (2-500 ppm) for all analytes, with a correlation coefficient of >0.998. The proposed method was found to be specific and precise for the quantification of shikonin derivatives in species.

Conclusion: The highest content of shikonin derivatives was observed in L. compared with the other species studied herein, advocating the use of L. roots as an alternate source for shikonin derivatives.

Citing Articles

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Establishment of the hairy root culture of L. and its shikonin production.

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