Magnetic Lanthanum-doped Hydroxyapatite/chitosan Scaffolds with Endogenous Stem Cell-recruiting and Immunomodulatory Properties for Bone Regeneration
Overview
Affiliations
Generally, the addition of exogenous stem cells and host-to-scaffold immune responses restricts the clinical applications of hydroxyapatite (HA)/chitosan (CS) scaffolds for bone regeneration. To achieve "facilitated endogenous tissue engineering", magnetic M-type hexagonal ferrite (SrFe12O19) nanoparticles were incorporated into bone scaffolds to recruit endogenous stem cells. Then, lanthanum incorporation was utilized to regulate host-to-scaffold immune responses and to provide a pro-regenerative environment for recruited endogenous stem cells. Here, we first fabricated and characterized magnetic lanthanum-doped HA/CS scaffolds. The MLaHA/CS scaffolds were demonstrated to be effective at recruiting rat bone marrow mesenchymal stem cells (rBMSCs) and modulating host-to-scaffold immune responses by promoting macrophage polarization into the anti-inflammatory phenotype (M2) in vitro. By further examining the underlying mechanism, we found that MLaHA/CS scaffolds could promote the osteogenic differentiation of rBMSCs by upregulating the phosphorylation of the Smad 1/5/9 pathway. When MLaHA/CS scaffolds were implanted into rat calvarial defects, the incorporation of magnetic nanoparticles and lanthanum significantly promoted the new bone regeneration, as revealed by micro-CT assays and histological staining. Our findings suggest that MLaHA/CS shows great potential for use as a cell-free and biocompatible scaffold for bone regeneration.
Sun Y, Wu S, Jiao Z, Liu D, Li X, Shang T Sci Rep. 2025; 15(1):6729.
PMID: 40000666 PMC: 11862006. DOI: 10.1038/s41598-025-90638-4.
Furlani F, Malfatti M, Rondinella A, Campodoni E, Sandri M, Fedrizzi L J Biol Eng. 2024; 18(1):60.
PMID: 39456111 PMC: 11515322. DOI: 10.1186/s13036-024-00458-9.
Farzamfar S, Melo Garcia L, Rahmani M, Bolduc S Bioengineering (Basel). 2024; 11(5).
PMID: 38790361 PMC: 11118848. DOI: 10.3390/bioengineering11050494.
He L J Funct Biomater. 2024; 15(4).
PMID: 38667541 PMC: 11050949. DOI: 10.3390/jfb15040084.
Chen Z, Zhou X, Mo M, Hu X, Liu J, Chen L J Nanobiotechnology. 2024; 22(1):185.
PMID: 38627717 PMC: 11020458. DOI: 10.1186/s12951-024-02442-3.