Quantification of Intestinal Species in Children with Functional Constipation by Quantitative Real-Time PCR

Overview

Journal Clin Exp Gastroenterol

Publisher Dove Medical Press

Specialty Gastroenterology

Date 2020 May 23

PMID 32440191

Citations 16

Authors

Nabi Jomehzadeh

Hazhir Javaherizadeh

Mansour Amin

Mohammad Rashno

Ali Teimoori

Affiliations

Soon will be listed here.

Abstract

Background: Functional constipation is a common and annoying gastrointestinal disorder among children worldwide in which the intestinal microbiota composition plays a fundamental role. This study aimed to compare the quantity of main intestinal species in constipated children and healthy controls.

Materials And Methods: Fecal samples were collected from 40 children fulfilling Rome IV criteria for functional constipation and 40 healthy volunteers. Quantitative real-time polymerase chain reaction (qPCR) method with species-specific primers was used to investigate seven main species in fecal samples.

Results: strains of the patient group were different from the healthy controls, main differences being noticeable decrease in the population quantity of (mean 10 CFU/gram feces) and lower abundance of (p<0.0001).

Conclusion: species might be quantitatively altered in childhood functional constipation. Our findings on the different species of population showed significantly decreased quantity in the patient group compared with the healthy subjects.

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