Two Light Responsive Genes Exhibit Positive and Negative Correlation with Picroside Content in Royle Ex Benth, an Endangered Medicinal Herb

Overview

Journal 3 Biotech

Publisher Springer

Specialty Biotechnology

Date 2020 May 21

PMID 32432017

Authors

Tanvi Sharma

Tabasum Kawoosa

Parul Gahlan

Damini Sharma

Anish Kaachra

Vipin Hallan

Sanjay Kumar

Affiliations

Soon will be listed here.

Abstract

is an endangered herb known to produce the medicinally important picrosides through isoprenoid pathway. The present work showed the functionality of WRKY motifs (TGAC -acting elements) present in the promoters of regulatory genes 3-hydroxy-3-methylglutaryl coenzyme A reductase () and 1-deoxy-d-xylulose-5-phosphate synthase () of the picrosides biosynthetic pathway by electrophoretic mobility shift assay. Also, the two genes, and were characterized and found to contain double and single characteristic WRKY domains, respectively along with a zinc-finger motif in each domain. Expression analysis revealed that and exhibited a positive and negative correlation, respectively, with picrosides content under the environment of light and in different tissues. Functional evaluation in yeast showed DNA binding ability of both PksWRKY and PkdWRKY; however, only PkdWRKY exhibited transcriptional activation ability. Transient overexpression of and in tobacco modulated the expression of selected native genes of tobacco involved in MVA and MEP pathway suggesting functionality of and in planta. Collectively, data suggested that and might be positive and negative regulators, respectively in the picrosides biosynthetic pathway.

References

Wang J, Tao F, An F, Zou Y, Tian W, Chen X . Wheat transcription factor TaWRKY70 is positively involved in high-temperature seedling plant resistance to Puccinia striiformis f. sp. tritici. Mol Plant Pathol. 2016; 18(5):649-661. PMC: 6638234. DOI: 10.1111/mpp.12425. View

Park C, Shin Y, Lee B, Kim K, Kim J, Paek K . A hot pepper gene encoding WRKY transcription factor is induced during hypersensitive response to Tobacco mosaic virus and Xanthomonas campestris. Planta. 2005; 223(2):168-79. DOI: 10.1007/s00425-005-0067-1. View

Zhang Z, Shin M, Zou X, Huang J, Ho T, Shen Q . A negative regulator encoded by a rice WRKY gene represses both abscisic acid and gibberellins signaling in aleurone cells. Plant Mol Biol. 2009; 70(1-2):139-51. DOI: 10.1007/s11103-009-9463-4. View

Li S, Zhang P, Zhang M, Fu C, Yu L . Functional analysis of a WRKY transcription factor involved in transcriptional activation of the DBAT gene in Taxus chinensis. Plant Biol (Stuttg). 2012; 15(1):19-26. DOI: 10.1111/j.1438-8677.2012.00611.x. View

Jiang W, Yu D . Arabidopsis WRKY2 transcription factor mediates seed germination and postgermination arrest of development by abscisic acid. BMC Plant Biol. 2009; 9:96. PMC: 2719644. DOI: 10.1186/1471-2229-9-96. View

Dai X, Wang Y, Zhang W . OsWRKY74, a WRKY transcription factor, modulates tolerance to phosphate starvation in rice. J Exp Bot. 2015; 67(3):947-60. PMC: 4737085. DOI: 10.1093/jxb/erv515. View

Lacomme C, Chapman S . Use of potato virus X (PVX)-based vectors for gene expression and virus-induced gene silencing (VIGS). Curr Protoc Microbiol. 2008; Chapter 16:Unit 16I.1. DOI: 10.1002/9780471729259.mc16i01s8. View

Zhang Z, Xie Z, Zou X, Casaretto J, Ho T, Shen Q . A rice WRKY gene encodes a transcriptional repressor of the gibberellin signaling pathway in aleurone cells. Plant Physiol. 2004; 134(4):1500-13. PMC: 419826. DOI: 10.1104/pp.103.034967. View

Lagace M, Matton D . Characterization of a WRKY transcription factor expressed in late torpedo-stage embryos of Solanum chacoense. Planta. 2004; 219(1):185-9. DOI: 10.1007/s00425-004-1253-2. View

10.

Printen J, Sprague Jr G . Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics. 1994; 138(3):609-19. PMC: 1206212. DOI: 10.1093/genetics/138.3.609. View

11.

Rastogi R, Srivastava A, Rastogi A . Long term effect of aflatoxin B(1) on lipid peroxidation in rat liver and kidney: effect of picroliv and silymarin. Phytother Res. 2001; 15(4):307-10. DOI: 10.1002/ptr.722. View

12.

Klok E, Wilson I, Wilson D, Chapman S, Ewing R, Somerville S . Expression profile analysis of the low-oxygen response in Arabidopsis root cultures. Plant Cell. 2002; 14(10):2481-94. PMC: 151230. DOI: 10.1105/tpc.004747. View

13.

Li P, Matsunaga K, Yamakuni T, Ohizumi Y . Picrosides I and II, selective enhancers of the mitogen-activated protein kinase-dependent signaling pathway in the action of neuritogenic substances on PC12D cells. Life Sci. 2002; 71(15):1821-35. DOI: 10.1016/s0024-3205(02)01949-5. View

14.

Calkhoven C, Ab G . Multiple steps in the regulation of transcription-factor level and activity. Biochem J. 1996; 317 ( Pt 2):329-42. PMC: 1217492. DOI: 10.1042/bj3170329. View

15.

Husain G, Singh P, Kumar V . Antidiabetic activity of standardized extract of Picrorhiza kurroa in rat model of NIDDM. Drug Discov Ther. 2012; 3(3):88-92. View

16.

Kawoosa T, Singh H, Kumar A, Sharma S, Devi K, Dutt S . Light and temperature regulated terpene biosynthesis: hepatoprotective monoterpene picroside accumulation in Picrorhiza kurrooa. Funct Integr Genomics. 2010; 10(3):393-404. DOI: 10.1007/s10142-009-0152-9. View

17.

Li S, Fu Q, Chen L, Huang W, Yu D . Arabidopsis thaliana WRKY25, WRKY26, and WRKY33 coordinate induction of plant thermotolerance. Planta. 2011; 233(6):1237-52. DOI: 10.1007/s00425-011-1375-2. View

18.

Wang Y, Guo D, Li H, Peng S . Characterization of HbWRKY1, a WRKY transcription factor from Hevea brasiliensis that negatively regulates HbSRPP. Plant Physiol Biochem. 2013; 71:283-9. DOI: 10.1016/j.plaphy.2013.07.020. View

19.

Han J, Wang H, Lundgren A, Brodelius P . Effects of overexpression of AaWRKY1 on artemisinin biosynthesis in transgenic Artemisia annua plants. Phytochemistry. 2014; 102:89-96. DOI: 10.1016/j.phytochem.2014.02.011. View

20.

Zheng Z, Mosher S, Fan B, Klessig D, Chen Z . Functional analysis of Arabidopsis WRKY25 transcription factor in plant defense against Pseudomonas syringae. BMC Plant Biol. 2007; 7:2. PMC: 1780049. DOI: 10.1186/1471-2229-7-2. View