Investigation of Fertilizing Capacity of Zebrafish () Sperm Exposed to Heavy Metals

Overview

Journal Dose Response

Publisher Sage Publications

Date 2020 May 20

PMID 32425723

Authors

Flora Kerekes

Timea Kollar

Gyongyi Gazsi

Eszter Kasa

Bela Urbanyi

Zsolt Csenki-Bakos

Akos Horvath

Affiliations

Soon will be listed here.

Abstract

The objective of our study was to investigate the effects of heavy metals on the fertilizing capacity of exposed zebrafish sperm, on embryonic survival, and on occurrence of embryonic deformities following fertilization with exposed sperm. It is important to test heavy metals because they are well-known pollutants. Sperm of externally fertilizing species can get in contact with pollutants found in aquatic environment. Zebrafish sperm, despite its advantages, has seldom been used in in vitro toxicological studies and no reports are available regarding the fertilizing capacity of exposed sperm. Zebrafish sperm was stripped and exposed to concentrations of the tested heavy metals (Zn, Cd, Cr, Cu, Ni, Hg, As) for 30 or 120 minutes. Calculated half-maximal effective concentration (EC) values do not differ significantly from those calculated for motility for any of the tested heavy metals, which means fertilization rate can indicate the toxicity of the given substance following exposure of sperm. Thus, its application as in vitro toxicological end point is reasonable. The survival of embryos and embryonic development have not been affected by the exposure of spermatozoa, which means all alterations in spermatozoa caused by heavy metals have been expressed before 24 hours post fertilization.

References

Ebrahimi M . Effects of in vivo and in vitro zinc and cadmium treatment on sperm steroidogenesis of the African catfish Clarias gairepinus. Pak J Biol Sci. 2008; 10(17):2862-7. DOI: 10.3923/pjbs.2007.2862.2867. View

Rurangwa E, Biegniewska A, Slominska E, Skorkowski E, Ollevier F . Effect of tributyltin on adenylate content and enzyme activities of teleost sperm: a biochemical approach to study the mechanisms of toxicant reduced spermatozoa motility. Comp Biochem Physiol C Toxicol Pharmacol. 2002; 131(3):335-44. DOI: 10.1016/s1532-0456(02)00019-4. View

Wang H, Liang Y, Li S, Chang J . Acute toxicity, respiratory reaction, and sensitivity of three cyprinid fish species caused by exposure to four heavy metals. PLoS One. 2013; 8(6):e65282. PMC: 3670833. DOI: 10.1371/journal.pone.0065282. View

Krasznai Z, Marian T, Izumi H, Damjanovich S, Balkay L, Tron L . Membrane hyperpolarization removes inactivation of Ca2+ channels, leading to Ca2+ influx and subsequent initiation of sperm motility in the common carp. Proc Natl Acad Sci U S A. 2000; 97(5):2052-7. PMC: 15752. DOI: 10.1073/pnas.040558097. View

Harrison R, Dott H, FOSTER G . Bovine serum albumin, sperm motility, and the "dilution effect'. J Exp Zool. 1982; 222(1):81-8. DOI: 10.1002/jez.1402220111. View

Hatef A, Alavi S, Golshan M, Linhart O . Toxicity of environmental contaminants to fish spermatozoa function in vitro--a review. Aquat Toxicol. 2013; 140-141:134-44. DOI: 10.1016/j.aquatox.2013.05.016. View

Li X, Wang P, Feng C, Liu D, Chen J, Wu F . Acute Toxicity and Hazardous Concentrations of Zinc to Native Freshwater Organisms Under Different pH Values in China. Bull Environ Contam Toxicol. 2018; 103(1):120-126. PMC: 6647607. DOI: 10.1007/s00128-018-2441-2. View

Kollar T, Kasa E, Ferincz A, Urbanyi B, Csenki-Bakos Z, Horvath A . Development of an in vitro toxicological test system based on zebrafish (Danio rerio) sperm analysis. Environ Sci Pollut Res Int. 2018; 25(15):14426-14436. DOI: 10.1007/s11356-018-1613-2. View

Kutluyer F, Erisir M, Benzer F, Ogretmen F, Evren Inanan B . The in vitro effect of Lambda-cyhalothrin on quality and antioxidant responses of rainbow trout Oncorhynchus mykiss spermatozoa. Environ Toxicol Pharmacol. 2015; 40(3):855-60. DOI: 10.1016/j.etap.2015.09.018. View

10.

Khan A, Weis J . Effects of methylmercury on sperm and egg viability of two populations of killifish (Fundulus heteroclitus). Arch Environ Contam Toxicol. 1987; 16(4):499-505. DOI: 10.1007/BF01055273. View

11.

Li Z, Li P, Rodina M, Randak T . Effect of human pharmaceutical Carbamazepine on the quality parameters and oxidative stress in common carp (Cyprinus carpio L.) spermatozoa. Chemosphere. 2010; 80(5):530-4. DOI: 10.1016/j.chemosphere.2010.04.046. View

12.

Li Z, Li P, Rodina M, Randak T . Evaluating the function of calcium antagonist on the Cd-induced stress in sperm of Russian sturgeon, Acipenser gueldenstaedtii. Aquat Toxicol. 2010; 100(4):373-5. DOI: 10.1016/j.aquatox.2010.08.007. View

13.

Rosety M, Ordonez F, Rosety-Rodriguez M, Rosety J, Rosety I . In vitro acute toxicity of anionic surfactant linear alkylbenzene sulphonate (LAS) on the motility of gilthead (Sparus aurata L.) sperm. Histol Histopathol. 2003; 18(2):475-8. DOI: 10.14670/HH-18.475. View

14.

Acosta I, Junior A, Fernandes E Silva E, Cardoso T, Caldas J, Jardim R . Effects of exposure to cadmium in sperm cells of zebrafish, . Toxicol Rep. 2017; 3:696-700. PMC: 5616012. DOI: 10.1016/j.toxrep.2016.08.002. View

15.

Zhou B, Liu W, Siu W, OToole D, Lam P, Wu R . Exposure of spermatozoa to duroquinone may impair reproduction of the common carp (Cyprinus carpio) through oxidative stress. Aquat Toxicol. 2006; 77(2):136-42. DOI: 10.1016/j.aquatox.2005.11.006. View

16.

Alsop D, Wood C . Metal uptake and acute toxicity in zebrafish: common mechanisms across multiple metals. Aquat Toxicol. 2011; 105(3-4):385-93. DOI: 10.1016/j.aquatox.2011.07.010. View

17.

Au D, Chiang M, Wu R . Effects of cadmium and phenol on motility and ultrastructure of sea urchin and mussel spermatozoa. Arch Environ Contam Toxicol. 2000; 38(4):455-63. DOI: 10.1007/s002449910060. View

18.

Lahnsteiner F, Berger B, Grubinger F, Weismann T . The effect of 4-nonylphenol on semen quality, viability of gametes, fertilization success, and embryo and larvae survival in rainbow trout (Oncorhynchus mykiss). Aquat Toxicol. 2005; 71(4):297-306. DOI: 10.1016/j.aquatox.2004.11.007. View

19.

Dietrich G, Dietrich M, Kowalski R, Dobosz S, Karol H, Demianowicz W . Exposure of rainbow trout milt to mercury and cadmium alters sperm motility parameters and reproductive success. Aquat Toxicol. 2010; 97(4):277-84. DOI: 10.1016/j.aquatox.2009.12.010. View

20.

Nagy S, Kakasi B, Bercsenyi M . Flow cytometric detection of oxidative DNA damage in fish spermatozoa exposed to cadmium - Short communication. Acta Vet Hung. 2016; 64(1):120-4. DOI: 10.1556/004.2016.013. View