ZSCAN4 Mouse Embryonic Stem Cells Have an Oxidative and Flexible Metabolic Profile
Overview
Authors
Affiliations
Cultured mouse embryonic stem cells are a heterogeneous population with diverse differentiation potential. In particular, the subpopulation marked by Zscan4 expression has high stem cell potency and shares with 2 cell stage preimplantation embryos both genetic and epigenetic mechanisms that orchestrate zygotic genome activation. Although embryonic de novo genome activation is known to rely on metabolites, a more extensive metabolic characterization is missing. Here we analyze the Zscan4 mouse stem cell metabolic phenotype associated with pluripotency maintenance and cell reprogramming. We show that Zscan4 cells have an oxidative and adaptable metabolism, which, on one hand, fuels a high bioenergetic demand and, on the other hand, provides intermediate metabolites for epigenetic reprogramming. Our findings enhance our understanding of the metastable Zscan4 stem cell state with potential applications in regenerative medicine.
Agriesti F, Landini F, Tamma M, Pacelli C, Mazzoccoli C, Calice G Stem Cell Res Ther. 2023; 14(1):215.
PMID: 37608350 PMC: 10463344. DOI: 10.1186/s13287-023-03447-9.
Identification of and as New Prame-Target Genes in 2C-like Embryonic Stem Cells.
Lucci V, De Marino E, Tagliaferri D, Amente S, Pollice A, Calabro V Genes (Basel). 2022; 13(10).
PMID: 36292630 PMC: 9601988. DOI: 10.3390/genes13101745.
Gastric Cancer Stem Cells: A Glimpse on Metabolic Reprogramming.
Addeo M, Di Paola G, Verma H, Laurino S, Russi S, Zoppoli P Front Oncol. 2021; 11:698394.
PMID: 34249759 PMC: 8262334. DOI: 10.3389/fonc.2021.698394.
ZSCAN4 mouse embryonic stem cells have an oxidative and flexible metabolic profile.
Troiano A, Pacelli C, Ruggieri V, Scrima R, Addeo M, Agriesti F EMBO Rep. 2020; 21(6):e48942.
PMID: 32424995 PMC: 7271321. DOI: 10.15252/embr.201948942.