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ZSCAN4 Mouse Embryonic Stem Cells Have an Oxidative and Flexible Metabolic Profile

Abstract

Cultured mouse embryonic stem cells are a heterogeneous population with diverse differentiation potential. In particular, the subpopulation marked by Zscan4 expression has high stem cell potency and shares with 2 cell stage preimplantation embryos both genetic and epigenetic mechanisms that orchestrate zygotic genome activation. Although embryonic de novo genome activation is known to rely on metabolites, a more extensive metabolic characterization is missing. Here we analyze the Zscan4 mouse stem cell metabolic phenotype associated with pluripotency maintenance and cell reprogramming. We show that Zscan4 cells have an oxidative and adaptable metabolism, which, on one hand, fuels a high bioenergetic demand and, on the other hand, provides intermediate metabolites for epigenetic reprogramming. Our findings enhance our understanding of the metastable Zscan4 stem cell state with potential applications in regenerative medicine.

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ZSCAN4 mouse embryonic stem cells have an oxidative and flexible metabolic profile.

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References
1.
Dan J, Yang J, Liu Y, Xiao A, Liu L . Roles for Histone Acetylation in Regulation of Telomere Elongation and Two-cell State in Mouse ES Cells. J Cell Physiol. 2015; 230(10):2337-44. PMC: 4711819. DOI: 10.1002/jcp.24980. View

2.
Troiano A, Pacelli C, Ruggieri V, Scrima R, Addeo M, Agriesti F . ZSCAN4 mouse embryonic stem cells have an oxidative and flexible metabolic profile. EMBO Rep. 2020; 21(6):e48942. PMC: 7271321. DOI: 10.15252/embr.201948942. View

3.
Li L, Zheng P, Dean J . Maternal control of early mouse development. Development. 2010; 137(6):859-70. PMC: 2834456. DOI: 10.1242/dev.039487. View

4.
Piantadosi C, Suliman H . Redox regulation of mitochondrial biogenesis. Free Radic Biol Med. 2012; 53(11):2043-53. PMC: 3604744. DOI: 10.1016/j.freeradbiomed.2012.09.014. View

5.
Shiraki N, Shiraki Y, Tsuyama T, Obata F, Miura M, Nagae G . Methionine metabolism regulates maintenance and differentiation of human pluripotent stem cells. Cell Metab. 2014; 19(5):780-94. DOI: 10.1016/j.cmet.2014.03.017. View