Optimization of an Extraction Solvent for Angiotensin-Converting Enzyme Inhibitors from L. Based on Its UPLC-MS/MS Metabolic Profiling

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2020 May 20

PMID 32422967

Citations 10

Authors

Mohamed A Salem

Haidy E Michel

Marwa I Ezzat

Mona M Okba

Ahmed M El-Desoky

Shanaz O Mohamed

Shahira M Ezzat

Affiliations

Soon will be listed here.

Abstract

species (Malvaceae) have been long used as an antihypertensive folk remedy. The aim of our study was to specify the optimum solvent for extraction of the angiotensin-converting enzyme inhibiting (ACEI) constituents from L. The 80% methanol extract (H2) showed the highest ACEI activity, which exceeds that of the standard captopril (IC50 0.01255 ± 0.00343 and 0.210 ± 0.005 µg/mL, respectively). Additionally, in a comprehensive metabolomics approach, an ultra-performance liquid chromatography (UPLC) coupled to the high resolution tandem mass spectrometry (HRMS) method was used to trace the metabolites from each extraction method. Interestingly, our comprehensive analysis showed that the 80% methanol extract was predominated with secondary metabolites from all classes including flavonoids, anthocyanins, phenolic and organic acids. Among the detected metabolites, phenolic acids such as ferulic and chlorogenic acids, organic acids such as citrate derivatives and flavonoids such as kaempferol have been positively correlated to the antihypertensive potential. These results indicates that these compounds may significantly contribute synergistically to the ACE inhibitory activity of the 80% methanol extract.

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