Tea Extract Increases Cell Fusion Via Regulation of Cell Surface DC-STAMP

Overview

Journal Biochem Biophys Rep

Specialty Biochemistry

Date 2020 May 19

PMID 32420461

Citations 3

Authors

Kenji Kuriya

Masahiro Nishio

Tomoko Matsuda

Hayato Umekawa

Affiliations

Soon will be listed here.

Abstract

Mononuclear osteoclast precursor cells fuse with each other to become mature multinucleated osteoclasts, which is regulated by dendritic cell-specific transmembrane protein (DC-STAMP). We evaluated the effects of tea extract and catechins on cell-cell fusion and DC-STAMP expression to elucidate their relationship with osteoclast development. When tea extract or epigallocatechin gallate (EGCg) was applied to RAW264.7 cells, multinucleated cells were increased significantly, while tartrate-resistant acid phosphatase (TRAP) activity was hardly upregulated. Flow cytometric analysis revealed that EGCg suppressed DC-STAMP expression on the cell surface, which is similar to osteoclast development. These observations suggest that TRAP activity is not activated even when suppression of both surface DC-STAMP expression and multinucleation occurs, which might be mediated by another pathway.

Citing Articles

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References

Yagi M, Ninomiya K, Fujita N, Suzuki T, Iwasaki R, Morita K . Induction of DC-STAMP by alternative activation and downstream signaling mechanisms. J Bone Miner Res. 2007; 22(7):992-1001. DOI: 10.1359/jbmr.070401. View

Shevde N, Anklesaria P, Greenberger J, Bleiberg I, Glowacki J . Stromal cell-mediated stimulation of osteoclastogenesis. Proc Soc Exp Biol Med. 1994; 205(4):306-15. DOI: 10.3181/00379727-205-43711. View

Cashman K . Diet, nutrition, and bone health. J Nutr. 2007; 137(11 Suppl):2507S-2512S. DOI: 10.1093/jn/137.11.2507S. View

Ilacqua A, Shettler J, Wernke K, Skalla J, McQuade K . Theaflavins from black tea affect growth, development, and motility in Dictyostelium discoideum. Biochem Biophys Res Commun. 2017; 491(2):449-454. DOI: 10.1016/j.bbrc.2017.07.058. View

Sobacchi C, Frattini A, Guerrini M, Abinun M, Pangrazio A, Susani L . Osteoclast-poor human osteopetrosis due to mutations in the gene encoding RANKL. Nat Genet. 2007; 39(8):960-2. DOI: 10.1038/ng2076. View

Hartgers F, Vissers J, Looman M, Van Zoelen C, Huffine C, Figdor C . DC-STAMP, a novel multimembrane-spanning molecule preferentially expressed by dendritic cells. Eur J Immunol. 2001; 30(12):3585-90. DOI: 10.1002/1521-4141(200012)30:12<3585::AID-IMMU3585>3.0.CO;2-Y. View

Staege H, Brauchlin A, Schoedon G, Schaffner A . Two novel genes FIND and LIND differentially expressed in deactivated and Listeria-infected human macrophages. Immunogenetics. 2001; 53(2):105-13. DOI: 10.1007/s002510100306. View

Vali B, Rao L, El-Sohemy A . Epigallocatechin-3-gallate increases the formation of mineralized bone nodules by human osteoblast-like cells. J Nutr Biochem. 2006; 18(5):341-7. DOI: 10.1016/j.jnutbio.2006.06.005. View

Yun J, Pang E, Kim C, Yoo Y, Cho K, Chai J . Inhibitory effects of green tea polyphenol (-)-epigallocatechin gallate on the expression of matrix metalloproteinase-9 and on the formation of osteoclasts. J Periodontal Res. 2004; 39(5):300-7. DOI: 10.1111/j.1600-0765.2004.00743.x. View

10.

Kukita T, Wada N, Kukita A, Kakimoto T, Sandra F, Toh K . RANKL-induced DC-STAMP is essential for osteoclastogenesis. J Exp Med. 2004; 200(7):941-6. PMC: 2213286. DOI: 10.1084/jem.20040518. View

11.

Parfitt A . The cellular basis of bone remodeling: the quantum concept reexamined in light of recent advances in the cell biology of bone. Calcif Tissue Int. 1984; 36 Suppl 1:S37-45. DOI: 10.1007/BF02406132. View

12.

Iwasaki R, Ninomiya K, Miyamoto K, Suzuki T, Sato Y, Kawana H . Cell fusion in osteoclasts plays a critical role in controlling bone mass and osteoblastic activity. Biochem Biophys Res Commun. 2008; 377(3):899-904. DOI: 10.1016/j.bbrc.2008.10.076. View

13.

Wu X, Xie C, Zhu Q, Wang M, Sun B, Huang Y . Green tea () aqueous extract alleviates postmenopausal osteoporosis in ovariectomized rats and prevents RANKL-induced osteoclastogenesis . Food Nutr Res. 2018; 62. PMC: 6190732. DOI: 10.29219/fnr.v62.1478. View

14.

Chen C, Ho M, Chang J, Hung S, Wang G . Green tea catechin enhances osteogenesis in a bone marrow mesenchymal stem cell line. Osteoporos Int. 2005; 16(12):2039-45. DOI: 10.1007/s00198-005-1995-0. View

15.

Mensah K, Ritchlin C, Schwarz E . RANKL induces heterogeneous DC-STAMP(lo) and DC-STAMP(hi) osteoclast precursors of which the DC-STAMP(lo) precursors are the master fusogens. J Cell Physiol. 2009; 223(1):76-83. PMC: 2814997. DOI: 10.1002/jcp.22012. View

16.

Morinobu A, Biao W, Tanaka S, Horiuchi M, Jun L, Tsuji G . (-)-Epigallocatechin-3-gallate suppresses osteoclast differentiation and ameliorates experimental arthritis in mice. Arthritis Rheum. 2008; 58(7):2012-8. DOI: 10.1002/art.23594. View

17.

Yagi M, Miyamoto T, Sawatani Y, Iwamoto K, Hosogane N, Fujita N . DC-STAMP is essential for cell-cell fusion in osteoclasts and foreign body giant cells. J Exp Med. 2005; 202(3):345-51. PMC: 2213087. DOI: 10.1084/jem.20050645. View

18.

Ko C, Lau K, Choy W, Leung P . Effects of tea catechins, epigallocatechin, gallocatechin, and gallocatechin gallate, on bone metabolism. J Agric Food Chem. 2009; 57(16):7293-7. DOI: 10.1021/jf901545u. View

19.

Chiu Y, Mensah K, Schwarz E, Ju Y, Takahata M, Feng C . Regulation of human osteoclast development by dendritic cell-specific transmembrane protein (DC-STAMP). J Bone Miner Res. 2011; 27(1):79-92. PMC: 3304467. DOI: 10.1002/jbmr.531. View

20.

. Assessment of fracture risk and its application to screening for postmenopausal osteoporosis. Report of a WHO Study Group. World Health Organ Tech Rep Ser. 1994; 843:1-129. View