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SARS-CoV-2 Detection by Direct RRT-PCR Without RNA Extraction

Overview
Journal J Clin Virol
Specialty Microbiology
Date 2020 May 17
PMID 32416598
Citations 63
Authors
Natacha Merindol
Genevieve Pepin
Caroline Marchand
Marylene Rheault
Christine Peterson
Andre Poirier
Claudia Houle
Hugo Germain
Alexis Danylo
Affiliations
Soon will be listed here.
Abstract

Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar® SARS-CoV-2 rRT-PCR kit (Altona Diagnostics). Furthermore, we assessed the impact of swab storage media composition on PCR efficiency. We show that SeeGene and Altona's assays provide similar efficiency. Importantly, we provide evidence that RNA extraction can be successfully bypassed when samples are stored in UTM medium or in molecular water but not when samples are stored in saline solution and in Hanks medium.

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References
1.
Corman V, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu D . Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020; 25(3). PMC: 6988269. DOI: 10.2807/1560-7917.ES.2020.25.3.2000045. View
2.
Wang D, Hu B, Hu C, Zhu F, Liu X, Zhang J . Clinical Characteristics of 138 Hospitalized Patients With 2019 Novel Coronavirus-Infected Pneumonia in Wuhan, China. JAMA. 2020; 323(11):1061-1069. PMC: 7042881. DOI: 10.1001/jama.2020.1585. View
3.
Chan J, Yip C, To K, Tang T, Wong S, Leung K . Improved Molecular Diagnosis of COVID-19 by the Novel, Highly Sensitive and Specific COVID-19-RdRp/Hel Real-Time Reverse Transcription-PCR Assay Validated and with Clinical Specimens. J Clin Microbiol. 2020; 58(5). PMC: 7180250. DOI: 10.1128/JCM.00310-20. View
4.
Moran A, Beavis K, Matushek S, Ciaglia C, Francois N, Tesic V . Detection of SARS-CoV-2 by Use of the Cepheid Xpert Xpress SARS-CoV-2 and Roche cobas SARS-CoV-2 Assays. J Clin Microbiol. 2020; 58(8). PMC: 7383516. DOI: 10.1128/JCM.00772-20. View
5.
Jin Y, Cai L, Cheng Z, Cheng H, Deng T, Fan Y . A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus (2019-nCoV) infected pneumonia (standard version). Mil Med Res. 2020; 7(1):4. PMC: 7003341. DOI: 10.1186/s40779-020-0233-6. View