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» Articles » PMID: 32409958

MiR-1224-5p Inhibits the Proliferation and Invasion of Ovarian Cancer Via Targeting SND1

Overview
Journal Hum Cell
Publisher Springer
Specialty Cell Biology
Date 2020 May 16
PMID 32409958
Citations 13
Authors
Junrong Wang
Yubo Hu
Cong Ye
Junbao Liu
Affiliations
Soon will be listed here.
Abstract

Emerging evidences have indicated that abnormal expression of microRNAs (miRNAs) contributed to carcinogenesis of ovarian cancer. However, the molecular mechanism of many aberrant expressed miRNAs was not known. Here, we discovered that miR-1224-5p was a downregulated miRNA in ovarian cancer via bioinformatic analysis and RT-qPCR. It was found that upregulation of miR-1224-5p inhibited cell proliferation and invasion ability of ovarian cancer cells. SND1, a well-characterized oncogene, was predicted as a target gene of miR-1224-5p. The western blotting, dual luciferase reporter assay, RNA-binding protein immunoprecipitation assay, and RT-qPCR demonstrated SND1 as a target gene of miR-1224-5p in ovarian cancer. MiR-1224-5p inhibited the expression of mesenchymal markers and increased the expression of epithelial markers in ovarian cancer cells via targeting SND1, indicating miR-1224-5p was involved in epithelial mesenchymal transition. The rescue assay manifested that miR-1224-5p-regulated cell proliferation and invasion mainly rely on downregulation of SND1 in ovarian cancer cells. In conclusion, our study revealed a direct regulatory association between miR-1224-5p and SND1 and their involvement in ovarian carcinogenesis.

Citing Articles

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Circ_0000115 Protects Against Cerebral Ischemia Injury by Suppressing Neuronal Apoptosis, Oxidative Stress and Inflammation by miR-1224-5p/Nos3 Axis In Vitro.

Cao Y, Xu Y, Zhang R, Qi J, Su Q, Chen Z Mol Biotechnol. 2023; 66(5):1082-1094.

PMID: 38151617 DOI: 10.1007/s12033-023-01005-5.

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Lin J, Wu Z, Zheng Y, Shen Z, Gan Z, Ma S Hum Genet. 2023; 143(9-10):1021-1034.

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Proteomic Discovery of Plasma Protein Biomarkers and Development of Models Predicting Prognosis of High-Grade Serous Ovarian Carcinoma.

Kim S, Hwangbo S, Dan K, Kim H, Chung H, Kim J Mol Cell Proteomics. 2023; 22(3):100502.

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Inhibition of miR-141-3p attenuates apoptosis of neural stem cells via targeting PBX1 to regulate PROK2 transcription in MCAO mice.

Liu T, Feng J, Sun Z, He M, Sun L, Dong S Cell Cycle. 2022; 22(4):403-418.

PMID: 36548024 PMC: 9879164. DOI: 10.1080/15384101.2022.2121358.

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