Generation of Recombinant VP3 Protein of Infectious Bursal Disease Virus in Three Different Expression Systems, Antigenic Analysis of the Obtained Polypeptides and Development of an ELISA Test

Overview

Journal Arch Virol

Specialty Microbiology

Date 2020 May 15

PMID 32405826

Citations 2

Authors

Dmitriy A Shirokov

Valentin A Manuvera

Olga A Miroshina

Alexandr S Dubovoi

Galina N Samuseva

Margarita E Dmitrieva

Vassili N Lazarev

Affiliations

Soon will be listed here.

Abstract

Infectious bursal disease virus (IBDV), which infects young chickens, is one of the most important pathogens that harm the poultry industry. Evaluation of the immune status of birds before and after vaccination is of great importance for controlling the disease caused by this virus. Therefore, the development of low-cost and easy-to-manufacture test systems for IBDV antibody detection remains an urgent issue. In this study, three expression systems (bacteria, yeast, and human cells) were used to produce recombinant VP3 protein of IBDV. VP3 is a group-specific antigen and hence may be a good candidate for use in diagnostic tests. Comparison of the antigenic properties of the obtained polypeptides showed that the titres of antibodies raised in chickens against bacteria- or human-cell-derived recombinant VP3 were high, whereas the antibody level against yeast-derived recombinant VP3 was low. The results of an enzyme-linked immunosorbent assay (ELISA) of sera from IBDV-infected chickens demonstrated that the recombinant VP3 produced in E. coli would be the best choice for use in test systems.

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