STAT4-mediated Down-regulation of MiR-3619-5p Facilitates Stomach Adenocarcinoma by Modulating TBC1D10B

Overview

Journal Cancer Biol Ther

Specialties Oncology
Pharmacology

Date 2020 May 14

PMID 32397798

Citations 7

Authors

Yinhua Liu

Jiaping Li

Sufeng Wang

Hong Song

Tao Yu

Affiliations

Soon will be listed here.

Abstract

Background: MicroRNAs (miRNAs) as the subtype of non-coding RNAs are revealed to be crucial players in cellular activities. It has been reported that miR-3619-5p functions as a tumor inhibitor in several cancers. However, the connection between miR-3619-5p and stomach adenocarcinoma (STAD) remains to be discovered.

Aim Of The Study: The purpose of the study is to figure out the role and molecular regulation mechanism of miR-3619-5p in STAD.

Methods: The expression of miR-3619-5p was evaluated via qRT-PCR analysis. Gain-of-function experiments demonstrated the effects of miR-3619-5p on cellular functions. The upper-stream transcription factor STAT4 and downstream target gene TBC1D10B of miR-3619-5p were identified by bioinformatic analysis. The binding and interaction between the indicated molecules were verified by RNA pull-down and luciferase reporter assays.

Results: The expression of miR-3619-5p was prominently down-regulated in STAD cells and tissues. MiR-3619-5p suppresses cell proliferation, migration, invasion and tumor growth in STAD. Further, STAT4 bound with miR-3619-5p promoter and inhibited its transcription. MiR-3619-5p was also recognized to modulate STAD progression through the regulation of downstream target gene TBC1D10B.

Conclusion: STAT4-mediated miR-3619-5p controls STAD carcinogenesis and progression through modulating TBC1D10B expression, which may provide a novel insight for researching the STAD-related molecular mechanism.

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