The Characterization of Structural Variations Within a Crystal Field
Overview
Affiliations
The technique of correlation averaging is refined by the use of multivariate statistical analysis and classification. The refined method can deal with the presence of structural variations within a crystal field. A low-dose image of a crotoxin crystal embedded in ice is used to demonstrate that crystallographic structural parameters characterizing the different areas of such a crystal with varying structure can be extracted rigorously and reproducibly.
Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM.
Righetto R, Biyani N, Kowal J, Chami M, Stahlberg H Nat Commun. 2019; 10(1):1722.
PMID: 30979902 PMC: 6461647. DOI: 10.1038/s41467-019-09661-5.
Outcome of the first electron microscopy validation task force meeting.
Henderson R, Sali A, Baker M, Carragher B, Devkota B, Downing K Structure. 2012; 20(2):205-14.
PMID: 22325770 PMC: 3328769. DOI: 10.1016/j.str.2011.12.014.
Single-particle reconstruction of biological macromolecules in electron microscopy--30 years.
Frank J Q Rev Biophys. 2009; 42(3):139-58.
PMID: 20025794 PMC: 2844734. DOI: 10.1017/S0033583509990059.
Frey W, Brink J, Schief Jr W, Chiu W, Vogel V Biophys J. 1998; 74(5):2674-9.
PMID: 9591691 PMC: 1299607. DOI: 10.1016/S0006-3495(98)77973-1.
Correlation analysis of gap junction lattice images.
Sosinsky G, Baker T, Caspar D, Goodenough D Biophys J. 1990; 58(5):1213-26.
PMID: 2291941 PMC: 1281066. DOI: 10.1016/S0006-3495(90)82462-0.