Designer Self-Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Overview

Journal Adv Sci (Weinh)

Specialty Biomedical Engineering

Date 2020 May 9

PMID 32382486

Citations 34

Authors

Zehong Yang

Hongyan Xu

Xiaojun Zhao

Affiliations

Soon will be listed here.

Abstract

Designer self-assembling peptides form the entangled nanofiber networks in hydrogels by ionic-complementary self-assembly. This type of hydrogel has realistic biological and physiochemical properties to serve as biomimetic extracellular matrix (ECM) for biomedical applications. The advantages and benefits are distinct from natural hydrogels and other synthetic or semisynthetic hydrogels. Designer peptides provide diverse alternatives of main building blocks to form various functional nanostructures. The entangled nanofiber networks permit essential compositional complexity and heterogeneity of engineering cell microenvironments in comparison with other hydrogels, which may reconstruct the tumor microenvironments (TMEs) in 3D cell cultures and tissue-specific modeling in vitro. Either ovarian cancer progression or recurrence and relapse are involved in the multifaceted TMEs in addition to mesothelial cells, fibroblasts, endothelial cells, pericytes, immune cells, adipocytes, and the ECM. Based on the progress in common hydrogel products, this work focuses on the diverse designer self-assembling peptide hydrogels for instructive cell constructs in tissue-specific modeling and the precise oncology remodeling for ovarian cancer, which are issued by several research aspects in a 3D context. The advantages and significance of designer peptide hydrogels are discussed, and some common approaches and coming challenges are also addressed in current complex tumor diseases.

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References

Huang H, Sun X . Rational design of responsive self-assembling peptides from native protein sequences. Biomacromolecules. 2010; 11(12):3390-4. DOI: 10.1021/bm100894j. View

Zhao F, Bae J, Zhou X, Guo Y, Yu G . Nanostructured Functional Hydrogels as an Emerging Platform for Advanced Energy Technologies. Adv Mater. 2018; 30(48):e1801796. DOI: 10.1002/adma.201801796. View

Koutsopoulos S . Self-assembling peptide nanofiber hydrogels in tissue engineering and regenerative medicine: Progress, design guidelines, and applications. J Biomed Mater Res A. 2015; 104(4):1002-16. DOI: 10.1002/jbm.a.35638. View

Rinoldi C, Costantini M, Kijenska-Gawronska E, Testa S, Fornetti E, Heljak M . Tendon Tissue Engineering: Effects of Mechanical and Biochemical Stimulation on Stem Cell Alignment on Cell-Laden Hydrogel Yarns. Adv Healthc Mater. 2019; 8(7):e1801218. DOI: 10.1002/adhm.201801218. View

Lampe K, Heilshorn S . Building stem cell niches from the molecule up through engineered peptide materials. Neurosci Lett. 2012; 519(2):138-46. PMC: 3691058. DOI: 10.1016/j.neulet.2012.01.042. View

Khoshakhlagh P, Moore M . Photoreactive interpenetrating network of hyaluronic acid and Puramatrix as a selectively tunable scaffold for neurite growth. Acta Biomater. 2015; 16:23-34. DOI: 10.1016/j.actbio.2015.01.014. View

Hong Y, Legge R, Zhang S, Chen P . Effect of amino acid sequence and pH on nanofiber formation of self-assembling peptides EAK16-II and EAK16-IV. Biomacromolecules. 2003; 4(5):1433-42. DOI: 10.1021/bm0341374. View

Redondo-Gomez C, Abdouni Y, Becer C, Mata A . Self-Assembling Hydrogels Based on a Complementary Host-Guest Peptide Amphiphile Pair. Biomacromolecules. 2019; 20(6):2276-2285. DOI: 10.1021/acs.biomac.9b00224. View

Wilson C, Bommarius A, Champion J, Chernoff Y, Lynn D, Paravastu A . Biomolecular Assemblies: Moving from Observation to Predictive Design. Chem Rev. 2018; 118(24):11519-11574. PMC: 6650774. DOI: 10.1021/acs.chemrev.8b00038. View

10.

Hoffman R . Clinical Correlation of the Histoculture Drug Response Assay in Gastrointestinal Cancer. Methods Mol Biol. 2018; 1760:61-72. DOI: 10.1007/978-1-4939-7745-1_7. View

11.

Pugliese R, Fontana F, Marchini A, Gelain F . Branched peptides integrate into self-assembled nanostructures and enhance biomechanics of peptidic hydrogels. Acta Biomater. 2017; 66:258-271. DOI: 10.1016/j.actbio.2017.11.026. View

12.

Zhang W, Ou X, Wu X . Proteomics profiling of plasma exosomes in epithelial ovarian cancer: A potential role in the coagulation cascade, diagnosis and prognosis. Int J Oncol. 2019; 54(5):1719-1733. PMC: 6438431. DOI: 10.3892/ijo.2019.4742. View

13.

Rose J, Gehlen D, Haraszti T, Kohler J, Licht C, De Laporte L . Biofunctionalized aligned microgels provide 3D cell guidance to mimic complex tissue matrices. Biomaterials. 2018; 163:128-141. DOI: 10.1016/j.biomaterials.2018.02.001. View

14.

Gao D, Vela I, Sboner A, Iaquinta P, Karthaus W, Gopalan A . Organoid cultures derived from patients with advanced prostate cancer. Cell. 2014; 159(1):176-187. PMC: 4237931. DOI: 10.1016/j.cell.2014.08.016. View

15.

Kreuzinger C, von der Decken I, Wolf A, Gamperl M, Koller J, Karacs J . Patient-derived cell line models revealed therapeutic targets and molecular mechanisms underlying disease progression of high grade serous ovarian cancer. Cancer Lett. 2019; 459:1-12. DOI: 10.1016/j.canlet.2019.05.032. View

16.

Peyton S, Gencoglu M, Galarza S, Schwartz A . Biomaterials in Mechano-oncology: Means to Tune Materials to Study Cancer. Adv Exp Med Biol. 2018; 1092:253-287. DOI: 10.1007/978-3-319-95294-9_13. View

17.

Choi S, Chaudhry P, Zo S, Han S . Advances in Protein-Based Materials: From Origin to Novel Biomaterials. Adv Exp Med Biol. 2018; 1078:161-210. DOI: 10.1007/978-981-13-0950-2_10. View

18.

Wu Y, Jia Z, Liu L, Zhao Y, Li H, Wang C . Functional Self-Assembled Peptide Nanofibers for Bone Marrow Mesenchymal Stem Cell Encapsulation and Regeneration in Nucleus Pulposus. Artif Organs. 2016; 40(6):E112-9. DOI: 10.1111/aor.12694. View

19.

Maru Y, Tanaka N, Itami M, Hippo Y . Efficient use of patient-derived organoids as a preclinical model for gynecologic tumors. Gynecol Oncol. 2019; 154(1):189-198. DOI: 10.1016/j.ygyno.2019.05.005. View

20.

Edri R, Gal I, Noor N, Harel T, Fleischer S, Adadi N . Personalized Hydrogels for Engineering Diverse Fully Autologous Tissue Implants. Adv Mater. 2018; 31(1):e1803895. DOI: 10.1002/adma.201803895. View