Transposable Elements Contribute to the Genome Plasticity of Species Complex

Overview

Journal Microb Genom

Specialties Genetics
Microbiology

Date 2020 May 8

PMID 32379020

Citations 9

Authors

Osiel Silva Goncalves

Kiara Franca Campos

Jessica Catarine Silva de Assis

Alexia Suellen Fernandes

Thamires Santos Souza

Luiz Guilherme do Carmo Rodrigues

Marisa Vieira de Queiroz

Mateus Ferreira Santana

Affiliations

Soon will be listed here.

Abstract

The extensive genetic diversity of , a serious soil-borne phytopathogen, has led to the concept that encompasses a species complex [ species complex (RSSC)]. Insertion sequences (ISs) are suggested to play an important role in the genome evolution of this pathogen. Here, we identified and analysed transposable elements (TEs), ISs and transposons, in 106 RSSC genomes and 15 spp. We mapped 10 259 IS elements in the complete genome of 62 representative RSSC strains and closely related spp. A unique set of 20 IS families was widespread across the strains, IS and IS being the most abundant. Our results showed six novel transposon sequences belonging to the Tn family carrying passenger genes encoding antibiotic resistance and avirulence proteins. In addition, internal rearrangement events associated with ISs were demonstrated in strains. We also mapped IS elements interrupting avirulence genes, which provided evidence that ISs plays an important role in virulence evolution of RSSC. Additionally, the activity of ISs was demonstrated by transcriptome analysis and DNA hybridization in isolates. Altogether, we have provided collective data of TEs in RSSC genomes, opening a new path for understanding their evolutionary impact on the genome evolution and diversity of this important plant pathogen.

Citing Articles

Investigating the impact of insertion sequences and transposons in the genomes of the most significant phytopathogenic bacteria.

Fernandes A, Campos K, de Assis J, Goncalves O, de Queiroz M, Bazzolli D Microb Genom. 2024; 10(4).

PMID: 38568199 PMC: 11092175. DOI: 10.1099/mgen.0.001219.

Chromosome-scale assembly uncovers genomic compartmentation of f. sp. , the causal agent of Bayoud disease in date palm.

Khayi S, Armitage A, Gaboun F, Meftah-Kadmiri I, Lahlali R, Fokar M Front Microbiol. 2023; 14:1268051.

PMID: 37886058 PMC: 10599148. DOI: 10.3389/fmicb.2023.1268051.

Influence of insertion sequences on population structure of phytopathogenic bacteria in the species complex.

Greenrod S, Stoycheva M, Elphinstone J, Friman V Microbiology (Reading). 2023; 169(7).

PMID: 37458734 PMC: 10433421. DOI: 10.1099/mic.0.001364.

Insights into the metabolic specificities of pathogenic strains from the species complex.

Baroukh C, Cottret L, Pires E, Peyraud R, Guidot A, Genin S mSystems. 2023; 8(4):e0008323.

PMID: 37341493 PMC: 10470067. DOI: 10.1128/msystems.00083-23.

Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants.

Pais A, Santos L, Albuquerque G, de Farias A, Silva Junior W, de Queiroz Balbino V Genet Mol Biol. 2022; 45(4):e20220038.

PMID: 36469480 PMC: 9731368. DOI: 10.1590/1678-4685-GMB-2022-0038.

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