Green Synthesis of Metallic Nanoparticles and Their Prospective Biotechnological Applications: an Overview

Overview

Journal Biol Trace Elem Res

Date 2020 May 8

PMID 32377944

Citations 239

Authors

Salem S Salem

Amr Fouda

Affiliations

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Abstract

The green synthesis of nanoparticles (NPs) using living cells is a promising and novelty tool in bionanotechnology. Chemical and physical methods are used to synthesize NPs; however, biological methods are preferred due to its eco-friendly, clean, safe, cost-effective, easy, and effective sources for high productivity and purity. High pressure or temperature is not required for the green synthesis of NPs, and the use of toxic and hazardous substances and the addition of external reducing, stabilizing, or capping agents are avoided. Intra- or extracellular biosynthesis of NPs can be achieved by numerous biological entities including bacteria, fungi, yeast, algae, actinomycetes, and plant extracts. Recently, numerous methods are used to increase the productivity of nanoparticles with variable size, shape, and stability. The different mechanical, optical, magnetic, and chemical properties of NPs have been related to their shape, size, surface charge, and surface area. Detection and characterization of biosynthesized NPs are conducted using different techniques such as UV-vis spectroscopy, FT-IR, TEM, SEM, AFM, DLS, XRD, zeta potential analyses, etc. NPs synthesized by the green approach can be incorporated into different biotechnological fields as antimicrobial, antitumor, and antioxidant agents; as a control for phytopathogens; and as bioremediative factors, and they are also used in the food and textile industries, in smart agriculture, and in wastewater treatment. This review will address biological entities that can be used for the green synthesis of NPs and their prospects for biotechnological applications.

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References

Elfeky A, Salem S, Elzaref A, Owda M, Eladawy H, Saeed A . Multifunctional cellulose nanocrystal /metal oxide hybrid, photo-degradation, antibacterial and larvicidal activities. Carbohydr Polym. 2020; 230:115711. DOI: 10.1016/j.carbpol.2019.115711. View

Dulinska-Molak I, Chlanda A, Li J, Wang X, Bystrzejewski M, Kawazoe N . The influence of carbon-encapsulated iron nanoparticles on elastic modulus of living human mesenchymal stem cells examined by atomic force microscopy. Micron. 2018; 108:41-48. DOI: 10.1016/j.micron.2018.02.006. View

Sharaf O, Al-Gamal M, Ibrahim G, Dabiza N, Salem S, El-Ssayad M . Evaluation and characterization of some protective culture metabolites in free and nano-chitosan-loaded forms against common contaminants of Egyptian cheese. Carbohydr Polym. 2019; 223:115094. DOI: 10.1016/j.carbpol.2019.115094. View

Hassan S, Fouda A, Radwan A, Salem S, Barghoth M, Awad M . Endophytic actinomycetes Streptomyces spp mediated biosynthesis of copper oxide nanoparticles as a promising tool for biotechnological applications. J Biol Inorg Chem. 2019; 24(3):377-393. DOI: 10.1007/s00775-019-01654-5. View

Shaheen T, Fouda A . Green approach for one-pot synthesis of silver nanorod using cellulose nanocrystal and their cytotoxicity and antibacterial assessment. Int J Biol Macromol. 2017; 106:784-792. DOI: 10.1016/j.ijbiomac.2017.08.070. View

Fouda A, Hassan S, Salem S, Shaheen T . In-Vitro cytotoxicity, antibacterial, and UV protection properties of the biosynthesized Zinc oxide nanoparticles for medical textile applications. Microb Pathog. 2018; 125:252-261. DOI: 10.1016/j.micpath.2018.09.030. View

Vidhu V, Philip D . Catalytic degradation of organic dyes using biosynthesized silver nanoparticles. Micron. 2013; 56:54-62. DOI: 10.1016/j.micron.2013.10.006. View

Kotil T, Akbulut C, Yon N . The effects of titanium dioxide nanoparticles on ultrastructure of zebrafish testis (Danio rerio). Micron. 2017; 100:38-44. DOI: 10.1016/j.micron.2017.04.006. View

Hashmi A, Rudolph M . Gold catalysis in total synthesis. Chem Soc Rev. 2008; 37(9):1766-75. DOI: 10.1039/b615629k. View

10.

Gawande M, Goswami A, Asefa T, Guo H, Biradar A, Peng D . Core-shell nanoparticles: synthesis and applications in catalysis and electrocatalysis. Chem Soc Rev. 2015; 44(21):7540-90. DOI: 10.1039/c5cs00343a. View

11.

Ulbrich K, Hola K, Subr V, Bakandritsos A, Tucek J, Zboril R . Targeted Drug Delivery with Polymers and Magnetic Nanoparticles: Covalent and Noncovalent Approaches, Release Control, and Clinical Studies. Chem Rev. 2016; 116(9):5338-431. DOI: 10.1021/acs.chemrev.5b00589. View

12.

Fathima J, Pugazhendhi A, Venis R . Synthesis and characterization of ZrO nanoparticles-antimicrobial activity and their prospective role in dental care. Microb Pathog. 2017; 110:245-251. DOI: 10.1016/j.micpath.2017.06.039. View

13.

Ahmad S, Munir S, Zeb N, Ullah A, Khan B, Ali J . Green nanotechnology: a review on green synthesis of silver nanoparticles - an ecofriendly approach. Int J Nanomedicine. 2019; 14:5087-5107. PMC: 6636611. DOI: 10.2147/IJN.S200254. View

14.

Grzelczak M, Perez-Juste J, Mulvaney P, Liz-Marzan L . Shape control in gold nanoparticle synthesis. Chem Soc Rev. 2008; 37(9):1783-91. DOI: 10.1039/b711490g. View

15.

Chen Y, Pepin A . Nanofabrication: conventional and nonconventional methods. Electrophoresis. 2001; 22(2):187-207. DOI: 10.1002/1522-2683(200101)22:2<187::AID-ELPS187>3.0.CO;2-0. View

16.

Mandal D, Bolander M, Mukhopadhyay D, Sarkar G, Mukherjee P . The use of microorganisms for the formation of metal nanoparticles and their application. Appl Microbiol Biotechnol. 2005; 69(5):485-92. DOI: 10.1007/s00253-005-0179-3. View

17.

Iravani S, Korbekandi H, Mirmohammadi S, Zolfaghari B . Synthesis of silver nanoparticles: chemical, physical and biological methods. Res Pharm Sci. 2015; 9(6):385-406. PMC: 4326978. View

18.

Boroumand Moghaddam A, Namvar F, Moniri M, Md Tahir P, Azizi S, Mohamad R . Nanoparticles Biosynthesized by Fungi and Yeast: A Review of Their Preparation, Properties, and Medical Applications. Molecules. 2015; 20(9):16540-65. PMC: 6332129. DOI: 10.3390/molecules200916540. View

19.

Gade A, Gaikwad S, Duran N, Rai M . Green synthesis of silver nanoparticles by Phoma glomerata. Micron. 2014; 59:52-9. DOI: 10.1016/j.micron.2013.12.005. View

20.

Banu A, Balasubramanian C . Optimization and synthesis of silver nanoparticles using Isaria fumosorosea against human vector mosquitoes. Parasitol Res. 2014; 113(10):3843-51. DOI: 10.1007/s00436-014-4052-0. View