Generation of a Gene Corrected Human Isogenic IBMS-iPSC-014-C from Polycystic-kidney-disease Induced Pluripotent Stem Cell Line Using CRISPR/Cas9

We report the engendering an isogenic iPSC line from the IBMS-iPSC-014-05 with homozygous correction of the R803X, Chr4: 88989098C > T in PKD2, using CRISPR/Cas9 technology. The results from the isogenic control, IBMS-iPSC-014-05C, showed that mutation had been corrected, while maintaining normal morphology, pluripotency, and differentiation capacity into three germ layers.