MicroRNA-520b Targets Interlukin-2 to Regulate the Growth, Metastasis and Drug Sensitivity of Liver Cancer Cells

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2020 May 2

PMID 32355540

Authors

Wei Sun

Wendong Li

Jinglong Chen

Ying Teng

Xiaoyan Ding

Affiliations

Soon will be listed here.

Abstract

Liver cancer is ranked as the 5 major type of cancer and is responsible for significant number of human deaths across the world. Recent investigations have shown microRNAs (miRs) to be involved in diverse cellular processes. Additionally, they have been shown to exhibit therapeutic implications in treatment of different diseases including cancer. This study investigated the role and therapeutic implications of miR-520b in liver cancer. The results of the present study revealed significant downregulation of miR-520b in liver cancer cell lines. Overexpression of miR-520b suppresses the growth of the SNU-182 and HepG2 cells by triggering apoptosis. This was also accompanied by upsurge of Bax and Caspase-3 and depletion of Bcl-2 in SNU-182 and HepG2 cells. Additionally, miR-520b caused a significant decrease in the migratory and invasive potential of the SNU-182 and HepG2 cells and enhanced their chemosensitivity to Gemcitabine. Bioinformatic analysis and the dual luciferase showed that miR-520b targets IL2 in HepG2 cells. Suppression of IL2 inhibits the growth of the HepG2 cells while as IL2 overexpression could avoid the tumor suppressive effects of miR-520b in HepG2 cells. Taken together, miR-520b may prove to be essential therapeutic target in liver cancer treatment and warrants further research endeavours.

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