Role of Cyclic Electron Transport Mutations Pgrl1 and Pgr5 in Acclimation Process to High Light in Chlamydomonas Reinhardtii

Overview

Journal Photosynth Res

Publisher Springer

Date 2020 May 1

PMID 32350701

Citations 6

Authors

Ranay Mohan Yadav

Sabit Mohammad Aslam

Sai Kiran Madireddi

Nisha Chouhan

Rajagopal Subramanyam

Affiliations

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Abstract

Light is crucial for photosynthesis, but the amount of light that exceeds an organism's assimilation efficacy can lead to photo-oxidative damage and even cell death. In Chlamydomonas (C). reinhardtii cyclic electron flow (CEF) is very important for the elicitation of non-photochemical quenching (NPQ) by controlling the acidification of thylakoid lumen. This process requires the cooperation of proton gradient regulation (PGR) proteins, PGRL1 and PGR5. Here, we compared the growth pattern and photosynthetic activity between wild type (137c, t222+) and mutants impaired in CEF (pgrl1 and pgr5) under photoautotrophic and photoheterotrophic conditions. We have observed the discriminative expression of NPQ in the mutants impaired in CEF of pgrl1 and pgr5. The results obtained from the mutants showed reduced cell growth and density, Chl a/b ratio, fluorescence, electron transport rate, and yield of photosystem (PS)II. These mutants have reduced capability to develop a strong NPQ indicating that the role of CEF is very crucial for photoprotection. Moreover, the CEF mutant exhibits increased photosensitivity compared with the wild type. Therefore, we suggest that besides NPQ, the fraction of non-regulated non-photochemical energy loss (NO) also plays a crucial role during high light acclimation despite a low growth rate. This low NPQ rate may be due to less influx of protons coming from the CEF in cases of pgrl1 and pgr5 mutants. These results are discussed in terms of the relative photoprotective benefit, related to the thermal dissipation of excess light in photoautotrophic and photoheterotrophic conditions.

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References

Allorent G, Tokutsu R, Roach T, Peers G, Cardol P, Girard-Bascou J . A dual strategy to cope with high light in Chlamydomonas reinhardtii. Plant Cell. 2013; 25(2):545-57. PMC: 3608777. DOI: 10.1105/tpc.112.108274. View

Alric J . Cyclic electron flow around photosystem I in unicellular green algae. Photosynth Res. 2010; 106(1-2):47-56. DOI: 10.1007/s11120-010-9566-4. View

DalCorso G, Pesaresi P, Masiero S, Aseeva E, Schunemann D, Finazzi G . A complex containing PGRL1 and PGR5 is involved in the switch between linear and cyclic electron flow in Arabidopsis. Cell. 2008; 132(2):273-85. DOI: 10.1016/j.cell.2007.12.028. View

Dang K, Plet J, Tolleter D, Jokel M, Cuine S, Carrier P . Combined increases in mitochondrial cooperation and oxygen photoreduction compensate for deficiency in cyclic electron flow in Chlamydomonas reinhardtii. Plant Cell. 2014; 26(7):3036-50. PMC: 4145130. DOI: 10.1105/tpc.114.126375. View

Derks A, Schaven K, Bruce D . Diverse mechanisms for photoprotection in photosynthesis. Dynamic regulation of photosystem II excitation in response to rapid environmental change. Biochim Biophys Acta. 2015; 1847(4-5):468-485. DOI: 10.1016/j.bbabio.2015.02.008. View

Erickson E, Wakao S, Niyogi K . Light stress and photoprotection in Chlamydomonas reinhardtii. Plant J. 2015; 82(3):449-465. DOI: 10.1111/tpj.12825. View

Fischer B, Wiesendanger M, Eggen R . Growth condition-dependent sensitivity, photodamage and stress response of Chlamydomonas reinhardtii exposed to high light conditions. Plant Cell Physiol. 2006; 47(8):1135-45. DOI: 10.1093/pcp/pcj085. View

Heifetz P, Forster B, Osmond C, Giles L, Boynton J . Effects of acetate on facultative autotrophy in Chlamydomonas reinhardtii assessed by photosynthetic measurements and stable isotope analyses. Plant Physiol. 2000; 122(4):1439-45. PMC: 58978. DOI: 10.1104/pp.122.4.1439. View

Hertle A, Blunder T, Wunder T, Pesaresi P, Pribil M, Armbruster U . PGRL1 is the elusive ferredoxin-plastoquinone reductase in photosynthetic cyclic electron flow. Mol Cell. 2013; 49(3):511-23. DOI: 10.1016/j.molcel.2012.11.030. View

10.

Johnson G . Physiology of PSI cyclic electron transport in higher plants. Biochim Biophys Acta. 2010; 1807(3):384-9. DOI: 10.1016/j.bbabio.2010.11.009. View

11.

Johnson X, Steinbeck J, Dent R, Takahashi H, Richaud P, Ozawa S . Proton gradient regulation 5-mediated cyclic electron flow under ATP- or redox-limited conditions: a study of ΔATpase pgr5 and ΔrbcL pgr5 mutants in the green alga Chlamydomonas reinhardtii. Plant Physiol. 2014; 165(1):438-52. PMC: 4012601. DOI: 10.1104/pp.113.233593. View

12.

Kalaji H, Schansker G, Brestic M, Bussotti F, Calatayud A, Ferroni L . Frequently asked questions about chlorophyll fluorescence, the sequel. Photosynth Res. 2016; 132(1):13-66. PMC: 5357263. DOI: 10.1007/s11120-016-0318-y. View

13.

Kodru S, Malavath T, Devadasu E, Nellaepalli S, Stirbet A, Subramanyam R . The slow S to M rise of chlorophyll a fluorescence reflects transition from state 2 to state 1 in the green alga Chlamydomonas reinhardtii. Photosynth Res. 2015; 125(1-2):219-31. DOI: 10.1007/s11120-015-0084-2. View

14.

Kramer D, Avenson T, Edwards G . Dynamic flexibility in the light reactions of photosynthesis governed by both electron and proton transfer reactions. Trends Plant Sci. 2004; 9(7):349-57. DOI: 10.1016/j.tplants.2004.05.001. View

15.

Kramer D, Johnson G, Kiirats O, Edwards G . New Fluorescence Parameters for the Determination of QA Redox State and Excitation Energy Fluxes. Photosynth Res. 2005; 79(2):209. DOI: 10.1023/B:PRES.0000015391.99477.0d. View

16.

Krieger-Liszkay A . Singlet oxygen production in photosynthesis. J Exp Bot. 2004; 56(411):337-46. DOI: 10.1093/jxb/erh237. View

17.

Miyachi S, Iwasaki I, Shiraiwa Y . Historical perspective on microalgal and cyanobacterial acclimation to low- and extremely high-CO(2) conditions. Photosynth Res. 2005; 77(2-3):139-53. DOI: 10.1023/A:1025817616865. View

18.

Munekage Y, Hojo M, Meurer J, Endo T, Tasaka M, Shikanai T . PGR5 is involved in cyclic electron flow around photosystem I and is essential for photoprotection in Arabidopsis. Cell. 2002; 110(3):361-71. DOI: 10.1016/s0092-8674(02)00867-x. View

19.

Munekage Y, Hashimoto M, Miyake C, Tomizawa K, Endo T, Tasaka M . Cyclic electron flow around photosystem I is essential for photosynthesis. Nature. 2004; 429(6991):579-82. DOI: 10.1038/nature02598. View

20.

Nama S, Madireddi S, Yadav R, Subramanyam R . Non-photochemical quenching-dependent acclimation and thylakoid organization of Chlamydomonas reinhardtii to high light stress. Photosynth Res. 2018; 139(1-3):387-400. DOI: 10.1007/s11120-018-0551-7. View