Acetaldehyde Production by Isolates from Patients with Oral Leukoplakia

Overview

Journal J Oral Microbiol

Specialty Dentistry

Date 2020 Apr 29

PMID 32341761

Citations 19

Authors

Abdrazak Amer

Aine Whelan

Nezar N Al-Hebshi

Claire M Healy

Gary P Moran

Affiliations

Soon will be listed here.

Abstract

has been found at high abundance on oral leukoplakia (OLK). The ability of clinical isolates to produce acetaldehyde (ACH) from ethanol has not been investigated. The objective of the current study was to determine the capacity of isolates recovered from OLK to generate ACH. Analysis of genomes (n = 70) shows that this species does not normally encode acetaldehyde dehydrogenase (ALDH) required for detoxification of ACH. The predicted OLK metagenome also exhibited reduced ALDH coding capacity. We analysed ACH production in 8 isolates of and showed that this species is capable of generating ACH in the presence of ethanol. The levels of ACH produced (mean = 53 µM) were comparable to those produced by and in parallel assays. These levels were demonstrated to induce oxidative stress in cultured oral keratinocytes. This study shows that can generate ACH from ethanol at levels which can induce oxidative stress. This organism likely contributes to oral ACH levels following alcohol consumption and the significance of the increased abundance of in patients with potentially malignant disorders requires further investigation.

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References

Lo J, Zheng T, Hon S, Olson D, Lynd L . The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is necessary for ethanol production in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum. J Bacteriol. 2015; 197(8):1386-93. PMC: 4372742. DOI: 10.1128/JB.02450-14. View

Homann N . Alcohol and upper gastrointestinal tract cancer: the role of local acetaldehyde production. Addict Biol. 2002; 6(4):309-323. DOI: 10.1080/13556210020077028. View

Gainza-Cirauqui M, Nieminen M, Novak Frazer L, Aguirre-Urizar J, Moragues M, Rautemaa R . Production of carcinogenic acetaldehyde by Candida albicans from patients with potentially malignant oral mucosal disorders. J Oral Pathol Med. 2012; 42(3):243-9. DOI: 10.1111/j.1600-0714.2012.01203.x. View

Yang X, Teng K, Su R, Li L, Zhang T, Fan K . AcrR and Rex Control Mannitol and Sorbitol Utilization through Their Cross-Regulation of Aldehyde-Alcohol Dehydrogenase (AdhE) in Lactobacillus plantarum. Appl Environ Microbiol. 2018; 85(4). PMC: 6365815. DOI: 10.1128/AEM.02035-18. View

Homann N, Tillonen J, Rintamaki H, Salaspuro M, Lindqvist C, Meurman J . Poor dental status increases acetaldehyde production from ethanol in saliva: a possible link to increased oral cancer risk among heavy drinkers. Oral Oncol. 2001; 37(2):153-8. DOI: 10.1016/s1368-8375(00)00076-2. View

Kobayashi T, Uchibori S, Tsuzukibashi O, Goto H, Aida M . A selective medium for Rothia mucilaginosa and its distribution in oral cavities. J Microbiol Methods. 2012; 91(3):364-5. DOI: 10.1016/j.mimet.2012.09.011. View

NOSOVA T, Jokelainen K, Kaihovaara P, Jousimies-Somer H, Siitonen A, Heine R . Aldehyde dehydrogenase activity and acetate production by aerobic bacteria representing the normal flora of human large intestine. Alcohol Alcohol. 1996; 31(6):555-64. DOI: 10.1093/oxfordjournals.alcalc.a008191. View

Nieminen M, Uittamo J, Salaspuro M, Rautemaa R . Acetaldehyde production from ethanol and glucose by non-Candida albicans yeasts in vitro. Oral Oncol. 2009; 45(12):e245-8. DOI: 10.1016/j.oraloncology.2009.08.002. View

Yokoyama S, Takeuchi K, Shibata Y, Kageyama S, Matsumi R, Takeshita T . Characterization of oral microbiota and acetaldehyde production. J Oral Microbiol. 2018; 10(1):1492316. PMC: 6041815. DOI: 10.1080/20002297.2018.1492316. View

10.

Muto M, Hitomi Y, Ohtsu A, Shimada H, Kashiwase Y, Sasaki H . Acetaldehyde production by non-pathogenic Neisseria in human oral microflora: implications for carcinogenesis in upper aerodigestive tract. Int J Cancer. 2000; 88(3):342-50. View

11.

Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett W . Metagenomic biomarker discovery and explanation. Genome Biol. 2011; 12(6):R60. PMC: 3218848. DOI: 10.1186/gb-2011-12-6-r60. View

12.

Dewhirst F, Chen T, Izard J, Paster B, Tanner A, Yu W . The human oral microbiome. J Bacteriol. 2010; 192(19):5002-17. PMC: 2944498. DOI: 10.1128/JB.00542-10. View

13.

Homann N, Tillonen J, Meurman J, Rintamaki H, Lindqvist C, Rautio M . Increased salivary acetaldehyde levels in heavy drinkers and smokers: a microbiological approach to oral cavity cancer. Carcinogenesis. 2000; 21(4):663-8. DOI: 10.1093/carcin/21.4.663. View

14.

Schmidt B, Kuczynski J, Bhattacharya A, Huey B, Corby P, Queiroz E . Changes in abundance of oral microbiota associated with oral cancer. PLoS One. 2014; 9(6):e98741. PMC: 4041887. DOI: 10.1371/journal.pone.0098741. View

15.

Kurkivuori J, Salaspuro V, Kaihovaara P, Kari K, Rautemaa R, Gronroos L . Acetaldehyde production from ethanol by oral streptococci. Oral Oncol. 2006; 43(2):181-6. DOI: 10.1016/j.oraloncology.2006.02.005. View

16.

Wattam A, Davis J, Assaf R, Boisvert S, Brettin T, Bun C . Improvements to PATRIC, the all-bacterial Bioinformatics Database and Analysis Resource Center. Nucleic Acids Res. 2016; 45(D1):D535-D542. PMC: 5210524. DOI: 10.1093/nar/gkw1017. View

17.

Connolly E, Millhouse E, Doyle R, Culshaw S, Ramage G, Moran G . The Porphyromonas gingivalis hemagglutinins HagB and HagC are major mediators of adhesion and biofilm formation. Mol Oral Microbiol. 2017; 32(1):35-47. DOI: 10.1111/omi.12151. View

18.

Perera M, Al-Hebshi N, Speicher D, Perera I, Johnson N . Emerging role of bacteria in oral carcinogenesis: a review with special reference to perio-pathogenic bacteria. J Oral Microbiol. 2016; 8:32762. PMC: 5039235. DOI: 10.3402/jom.v8.32762. View

19.

Marttila E, Uittamo J, Rusanen P, Lindqvist C, Salaspuro M, Rautemaa R . Acetaldehyde production and microbial colonization in oral squamous cell carcinoma and oral lichenoid disease. Oral Surg Oral Med Oral Pathol Oral Radiol. 2013; 116(1):61-8. DOI: 10.1016/j.oooo.2013.02.009. View

20.

Sanglard D, Ischer F, Monod M, Bille J . Cloning of Candida albicans genes conferring resistance to azole antifungal agents: characterization of CDR2, a new multidrug ABC transporter gene. Microbiology (Reading). 1997; 143 ( Pt 2):405-416. DOI: 10.1099/00221287-143-2-405. View